Team:DTU Denmark

Welcome to the DTU-DENMARK iGEM team wiki. Here we present everything about our project, the team, how we've contributed to improve the registry of standard biological parts, and much more.

Our team consists of six students in biotechnology and systems biology engineering, and we are the first team from the Technical University of Denmark. Please check out our team page to read more about us and our advisors.

In this project we have

• Designed and constructed a redox sensing device: The Redoxilator
  
• Written and submitted a novel biobrick assembly standard based on USER fusion that allows the assembly of several biobricks at once
  
• Made a program for the design of primers needed for USER fusion online: PHUSER
  

Read more about this in The Project

Furthermore we have constructed and submitted four very useful biobricks to the registry: Parts Submitted

Redox sensing device
Two novel genes have been designed and synthesized each comprised of 5 genetic elements. Together they function as a device termed the Redoxilator that can sense the internal redox state of a yeast cell, and output a reporter signal.

Biobricks
DNA of several new biobricks have been designed and submitted including a yeast optimized GFP reporter protein, a protein degradation sequence and a fast degradable yeast GFP. (Bronze medal)

We have demonstrated that our USER fusion biobrick works as expected and documented it (silver medal)

USER fusion Assembly standard
A new biobrick assembly standard that allows the rapid construction of multi-part devices have been developed and documented. The assembly standard offers many benefits: All restriction sites are allowed, multiple biobricks can be joined in one step, the result is scar-free making it ideal for protein fusions and more. (Gold medal)

USER-fusion primer design software
A novel and very useful software tool have been developed that can automatically design the optimal primers for USER fusion assembly of 2-9 biobricks, taking several parameters into account.