Team:IIT Madras/Results

Results

Comparing the differences in the growth rates of cells with and without plasmids in various media

Growth curves

Fig 8.1: DH5a grown in LB containing no antibiotic, LB containing Ampicillin, LB containing Chloramphenicol, LB containing both antibiotics.

Fig 8.2: RFP (pSB1C3) contanining cells grown in LB containing no antibiotic, LB containing Ampicillin, LB containing Chloramphenicol, LB containing both antibiotics.

Fig 8.3: CFP (pSB1A2) contanining cells grown in LB containing no antibiotic, LB containing Ampicillin, LB containing Chloramphenicol, LB containing both antibiotics.

Fig 8.4: RFP (pSB1C3) - CFP (pSB1A2) contanining cells grown in LB containing no antibiotic, LB containing Ampicillin, LB containing Chloramphenicol, LB containing both antibiotics.

Fig 8.5: All 4 strains in media without antibiotics.

Fig 8.6: All 4 strains in their corresponding antibiotic media - DH5a in Lb without antibiotics, RFP (pSB1C3) in LB with Chloramphenicol, CFP (pSB1A2) in LB with Ampicillin, RFP (pSB1C3) - CFP (pSB1A2) cells in LB with Chloramphenicol and Ampicillin.

Fig 8.7: log (OD600) vs Time plot for all 4 strains in media without antibiotics.

Fig 8.8: log (OD600) vs Time plot for all 4 strains in their corresponding antibiotic media - DH5a in Lb without antibiotics, RFP (pSB1C3) in LB with Chloramphenicol, CFP (pSB1A2) in LB with Ampicillin, RFP (pSB1C3) - CFP (pSB1A2) cells in LB with Chloramphenicol and Ampicillin.

Modeling

For updated results, keep checking here.

Fluorescent Imaging

The panel below shows cells co-transformed with both constructs, namely expressing CFP with Ampicillin and RFP with Chloramphenicol.

(a) shows cells exposed to Bright Field.

(b) shows cells exposed to Red filter.

(c) shows cells exposed to Cyan filter.

For updated results, keep checking here.

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