Team:LCG-UNAM-Mexico/Wet Lab/Objectives
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=='''Personal Objectives'''== | =='''Personal Objectives'''== | ||
+ | === Uriel === | ||
+ | |||
+ | -Construction of the phage production control system. | ||
+ | |||
+ | In order to produce a grate amount of P4 phage particles that has our death system, we want to avoid the natural | ||
+ | early lysis that occur when WT P4 and P2 interact. This avoidance will be achieved by taking the control of the two | ||
+ | major regulators of P2 morphopoietic genes. The control systems that is going to be implemented is constituted by a | ||
+ | promoter inducible by IPTG () in conjunction with transactivators cox and ogr from phage P2. All this will allow | ||
+ | us to grow bacteria in grate quantities and induce lysis when ever we want and as a consequence obtation of grate | ||
+ | amounts of P4 phage. | ||
=='''Work Journals'''== | =='''Work Journals'''== |
Revision as of 18:49, 21 October 2009