Team:UNICAMP-Brazil/Notebooks/October 15

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==''' ColiGuard '''==
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==== PY Promoter - Preparation of electrocompetent cells ====
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*<p style=”text-align:justify;”>Today we prepared electrocompetent cells of the conjugative strain following
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[https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Preparation_of_electrocompetent_E._coli Protocol 4].</p>

Revision as of 19:39, 21 October 2009

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ColiGuard

PY Promoter - Preparation of electrocompetent cells

  • Today we prepared electrocompetent cells of the conjugative strain following

Protocol 4.


YeastGuard

New strategy: pGEM

  • We sent 4 biobricks to iGEM today: pJEN1, pDLD, Lysozyme, and the devices Adh1+YFP and Adh1+Lysozyme. =)

Yeast experiments

  • We transformed the YEP+Adh1-lysozyme ligation reaction in competent E. coli and plated in LB+Amp media. Hope to have colonies later!

  • We did miniprep of YEP+Adh1-YFP and the following devices: b) pJEN1+YFP, c) pJEN1+Lys, d) pDLD+YFP, e) pDLD+Lys.

  • We did the pre inoculun to prepare competet yeast tomorrow.


Raíssa and Taís




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