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Team:ArtScienceBangalore/Parts
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<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Notebook">Notebook</a></li> | <li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Notebook">Notebook</a></li> | ||
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Outreach">Outreach</a></li> | <li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Outreach">Outreach</a></li> | ||
| + | <li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Experiments">Experiments</a></li> | ||
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Links">Links</a></li> | <li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Links">Links</a></li> | ||
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Gallery">Gallery</a></li> | <li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Gallery">Gallery</a></li> | ||
Revision as of 20:30, 21 October 2009
Parts
Here is the part that we submitted to the Standard Parts Registry-
BBa_K221000
This helps our construct synthesize geosmin, the enzyme that smells like rain.
This part codes N-terminal (366 amino acid) domain of the SCO6073 gene from S. coelicolor A3. Expression of this domain gives a fully functional germacradienol synthase with steady-state catalytic parameters similar to those of the full-length protein that converts farnesyl diphosphate(FPP) to Geosmin*.
Amino acid sequence:
MTQQPFQLPHFYLPHPARLNPHLDEARAHSTTWAREMGMLEGSGVWEQSDLEAHDYGLLCAYTHPDCDGPALSLITDWYVWVFFFDDHFLEK
YKRSQDRLAGKAHLDRLPLFMPLDDAAGMPEPRNPVEAGLADLWTRTVPAMSADWRRRFAVATEHLLNESMWELSNINEGRVANPVEYIEM
RRKVGGAPWSAGLVEYATAEVPAAVAGTRPLRVLMETFSDAVHLRNDLFSYQREVEDEGELSNGVLVLETFFGCTTQEAADLVNDVLTSRLH
QFEHTAFTEVPAVALEKGLTPLEVAAVGAYTKGLQDWQSGGHEWHMRSSRYMNKGERPLAGWQALTGPGTSAADVGALLADAVAQRARSYT
BBa_K221000
This helps our construct synthesize geosmin, the enzyme that smells like rain.
This part codes N-terminal (366 amino acid) domain of the SCO6073 gene from S. coelicolor A3. Expression of this domain gives a fully functional germacradienol synthase with steady-state catalytic parameters similar to those of the full-length protein that converts farnesyl diphosphate(FPP) to Geosmin*.
Amino acid sequence:
MTQQPFQLPHFYLPHPARLNPHLDEARAHSTTWAREMGMLEGSGVWEQSDLEAHDYGLLCAYTHPDCDGPALSLITDWYVWVFFFDDHFLEK
YKRSQDRLAGKAHLDRLPLFMPLDDAAGMPEPRNPVEAGLADLWTRTVPAMSADWRRRFAVATEHLLNESMWELSNINEGRVANPVEYIEM
RRKVGGAPWSAGLVEYATAEVPAAVAGTRPLRVLMETFSDAVHLRNDLFSYQREVEDEGELSNGVLVLETFFGCTTQEAADLVNDVLTSRLH
QFEHTAFTEVPAVALEKGLTPLEVAAVGAYTKGLQDWQSGGHEWHMRSSRYMNKGERPLAGWQALTGPGTSAADVGALLADAVAQRARSYT
