Team:LCG-UNAM-Mexico/Resources/Strains

E. coli Strains

Strains handed by Giuseppe Bertani

• C-1a.- Main strain for working with phage P2 and P4. This is also the mother strain for all the modified phage-containing derivatives. Its genetic map is really close to E. coli K12 "pop" strain. Its genome is being sequenced.

• C-117.- Lysogenic for P2

• C-1906.- Lysogenic for P4.

Strains handed by Richard Calendar

• C-331 /arg/-am/1/ /tonA-trp/D, str-r (P2) (P4 /imp/). The abbreviation "imp" means "improved plaques."

• C-520 F^+ /supD60/, used for growth and plaque assay of P2.

• C-1895 F^+ /supD60/ (P2 /lg/), which we use to grow and sometimes assay for P4. The symbol "lg" denotes large plaques.

• C-2423 /trp/-am /arg/-am/1/ str-r (P2 /lg/ del/1/). This strain is used to get the best P4 plaques. The symbol del1 denotes a

deletion of the /old /gene, which interferes with the growth of phage lambda and kills /recB-/ bacteria.

Strain variants obtained in our lab

• C-1895 sp+.- this slow-growing strain can be used for obtaining an excelent stock of P4 sid1

Expression Strains

BL21(DE3)pLysS: widely used for protein expression deficient in lon and ompT proteases. It has a chromosomal copy of the T7 RNA polymerase and T7 lysozyme for suppression o basal expression of T7 RNA polymerase and avoid as much as possible cell growth and viability affection.

Yeast strains

TERRIBLY, UNCOUNTABLE, PERSISTENT and ANTIBIOTIC RESISTANT!!!