Team:DTU Denmark/notebookredoxilator

From 2009.igem.org

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Revision as of 20:59, 21 October 2009

Welcome to the DTU iGEM wiki!

Notebook

Activities relating to our two sub-projects:

- The Redoxilator
- The USER^TM assembly standard
- Biobricks
- Protocols

Day-to-day activities

Activities relating to the redoxilator

Thursday 15/10/2009
BioLector experiment initiated! Lets get some results!

Wednesday 14/10/2009
To have inoculum for the BioLector experiment, colonies were picked from our plates and grown till tomorrow.

Monday 5/10/2009
The yeasts were streak diluted onto a new plate to be sure that we have a single transformant.

Thursday 1/10/2009
Our redoxilator construct and leaky construct were ready for yeast transformation into CEN PK 113 5D (Ura-) using the URA marker present these plasmids.

Wednesday 30/9/2009
The resulting vectors were purification. The presence of our leaky redoxilator construct was verified by digestion with XmaI.

Tuesday 29/9/2009
Liquid over night colonies were prepared. Cultures were randomly picked using a sterile pipette tip from the transformation plate and incubated into liquid media containing kanamycin.

Monday 28/9/2009
To test how leaky our redoxilator construct was, the redoxilator part was removed by NotI digested followed by gel-purification. The backbone missing the redox sensor/activator was ligated and transformed into E. coli.

Friday 25/9/2009
Colonies seen!

Thursday 24/9/2009
Our dear redoxilator construct arrieved from California (DNA 2.0) The DNA was extracted from filter paper using MilliQ water and transformed into E. Coli.

Work process

Our team works parallel in smaller sub-teams. Some of us work hard in the lab, while others are in the process of developing software and the in silico model of the Redoxilator system. However, we constantly keep each other updated, and meet often to exchange ideas and take turns at the different tasks, thus exhausting all of our combined knowledge in every aspect of this project.

Comments or questions to the team? Please Email us -- Comments of questions to webmaster? Please Email us

@@ Line 202: / Line 202: @@
 <font size="3"><b> Thursday 1/10/2009 </b></font><br>
-Our redoxilator construct and <i>leaky</i> construct were ready for yeast transformation into CEN PK 113 5D (Ura-) using the URA marker present these plasmids<br><br>
+Our redoxilator construct and <i>leaky</i> construct were ready for yeast transformation into CEN PK 113 5D (Ura-) using the URA marker present these plasmids.<br><br>
 <font size="3"><b> Wednesday 30/9/2009 </b></font><br>
-The resulting vectors were purification. The presence of our leaky redoxilator construct was verified by digestion with XmaI<br><br>
+The resulting vectors were purification. The presence of our leaky redoxilator construct was verified by digestion with XmaI.<br><br>
 <font size="3"><b> Tuesday 29/9/2009 </b></font><br>
@@ Line 212: / Line 212: @@
 <font size="3"><b> Monday 28/9/2009 </b></font><br>
-To test how leaky our redoxilator construct was, the redoxilator part was removed by NotI digested followed by gel-purification. The backbone missing the redox sensor/activator was ligated and transformed into <i>E. coli</i><br><br>
+To test how leaky our redoxilator construct was, the redoxilator part was removed by NotI digested followed by gel-purification. The backbone missing the redox sensor/activator was ligated and transformed into <i>E. coli</i>.<br><br>
@@ Line 220: / Line 220: @@
 <font size="3"><b> Thursday 24/9/2009 </b></font><br>
 Our dear redoxilator construct arrieved from California (DNA 2.0)
-The DNA was extracted from filter paper using MilliQ water and transformed into <i>E. Coli</i>
+The DNA was extracted from filter paper using MilliQ water and transformed into <i>E. Coli</i>.