Team:Michigan/Project
From 2009.igem.org
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==<B><font size=4>Suicide Mechanism</font></B>== | ==<B><font size=4>Suicide Mechanism</font></B>== | ||
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- | The Terminator's suicide mechanism, or kill switch, operates through the [http://partsregistry.org/wiki/index.php?title=Part:BBa_K112808<font color=navy><B>Enterobacteria phage T4 Lysis Device</B></font>] created by the Berkley 2008 team. We proposed two mechanisms for cell lysis: arabinose inducible suicide mechanism and suicide mechanism with tunable repression. In the both models the Pu promoter is controlling the cell survival. | + | The Terminator's suicide mechanism, or kill switch, operates through the [http://partsregistry.org/wiki/index.php?title=Part:BBa_K112808<font color=navy><B>Enterobacteria phage T4 Lysis Device</B></font>] created by the Berkley 2008 team. We proposed two mechanisms for cell lysis: arabinose inducible suicide mechanism and suicide mechanism with tunable repression. In the both models the Pu promoter is controlling the cell survival and we make the assumption that when the cells are used for bioremediation purposes they are placed in a toluene-contaminated environment. |
<B><font size=3>Arabinose Inducible Suicide Mechanism</font></B> | <B><font size=3>Arabinose Inducible Suicide Mechanism</font></B> | ||
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The motivation for this design is as follows. The Berkeley 2008 design used an inducible promoter in front of the holin and endolysin; this inducible promoter then would trigger the onset of the killing mechanism. In our design, we wanted to use Pu to make the killing mechanism responsive to toluene concentration. However, we needed to set this up such that Pu would prevent cell death. Due to this requirement, we designed an inverter that is comprised of the repression module. This way, we did not have to modify the Berkeley part, which is characterized as working. | The motivation for this design is as follows. The Berkeley 2008 design used an inducible promoter in front of the holin and endolysin; this inducible promoter then would trigger the onset of the killing mechanism. In our design, we wanted to use Pu to make the killing mechanism responsive to toluene concentration. However, we needed to set this up such that Pu would prevent cell death. Due to this requirement, we designed an inverter that is comprised of the repression module. This way, we did not have to modify the Berkeley part, which is characterized as working. | ||
+ | It should be noted that this design requires the cells to be stored in media containing substrates that would activate the Pu promoter (e.g. toluene, o-xylene, methyl-benzene)in order to transport the cells to the actual contaminated site. This is because the cells would die in the absence of such substrates. | ||
Revision as of 21:11, 21 October 2009
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