Team:Chiba/Project/reporter

(Difference between revisions)

Revision as of 00:11, 22 October 2009

In our expetriments, the more better condition of medium for the animation demonstration was exxamined with 5 different reporters.

Reporter	GFPuv	mRFP	sfGFP	mCherry	mOrange
Time of Recognizing the fastest LuxR [h]	2	3.5	4	5	11
Time of Recognizing the last LuxR [h]	3	24.5	50	25.5	71

Medium ：agar 5%, Thickness 7.0 mm

Temperature : r.t. (ca. 25 degree)

The photos were obtained at 0, 2, 3.5, 4, 5 h and several days after the incubation started.

GFPuv	mRFP	sfGFP	mCherry
mOrange

@@ Line 1: / Line 1: @@
-===Reporterに依る光り始める時刻の差===
+===Time delay depending on the reporters===
-今回培地の最もよい条件を調べる実験で5種のReporterを使用したが、それぞれのReporterを含む菌が光り始める時刻には、以下のような差がみられた。
+In our expetriments, the more better condition of medium for the animation demonstration was exxamined with 5 different reporters.
 <table width="502" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000"><tr>
@@ Line 13: / Line 12: @@
 </tr>
 <tr>
-<td>一番早いLuxRが光り始めた時刻 [h]</td>
+<td>Time of Recognizing the fastest LuxR [h]</td>
 <td></td>
 <td>2</td>
@@ Line 22: / Line 21: @@
 </tr>
 <tr>
-<td>一番遅いLuxRが光り始めた時刻 [h]</td>
+<td>Time of Recognizing the last LuxR [h]</td>
 <td></td>
 <td>3</td>
@@ Line 32: / Line 31: @@
 </table>
-*基礎データ
+*Condition
-培地：agar 5%, Thickness 7.0 mm
+Medium ：agar 5%, Thickness 7.0 mm
-温度：室温（25℃前後）
+Temperature : r.t. (ca. 25 degree)
-よって、Reporterを変えることで少なくとも4種のdelayを得ることができたと言える。
-*参考
+*The photos were obtained at 0, 2, 3.5, 4, 5 h and several days after the incubation started.
-上段左から0 h, 2 h,  3.5 h, 下段にいって4 h, 5 h, 十分時間が経ったあとの蛍光の様子、です。