Week18
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- | 1. Growth curve for specific growth rate determination of all the four strains on Minimal medium. | + | 1. Growth curve was done for specific growth rate determination of all the four strains on Minimal medium. |
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+ | 2. FACS was performed with all the four strains at different temperature to find out the effect of temperature | ||
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+ | on copy number of plasmid. | ||
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+ | 3. FACS was performed for different conc. of IPTG from O to 100 micro molar to observe the linearity of FACS | ||
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+ | expression. |
Latest revision as of 01:48, 22 October 2009
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1. Growth curve was done for specific growth rate determination of all the four strains on Minimal medium. 2. FACS was performed with all the four strains at different temperature to find out the effect of temperature on copy number of plasmid. 3. FACS was performed for different conc. of IPTG from O to 100 micro molar to observe the linearity of FACS expression. |