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Team:LCG-UNAM-Mexico/Wet Lab/Experiments
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====Multipromotor==== | ====Multipromotor==== | ||
| - | We will test the functionality of both T3 and T7 promoters trhough the induction with IPTG of the strain | + | We will test the functionality of both T3 and T7 promoters trhough the induction with IPTG of the strain [https://2009.igem.org/Team:LCG-UNAM-Mexico/Resources/Strains BL21(DE3)pLysS] in the case of T7. We are going to extract by PCR the RNA polymerase of the phage T3 and clone it under the control of a promoter inducible with IPTG. |
In both cases we expect to see the precense of fluorescence under uv light. | In both cases we expect to see the precense of fluorescence under uv light. | ||
Revision as of 22:10, 21 October 2009
Contents |
C1a growth plot
T3 and T7 infection plot
Without system
With system
Burst size determination
Without system
With system
Test parts and devices
Multipromotor
We will test the functionality of both T3 and T7 promoters trhough the induction with IPTG of the strain BL21(DE3)pLysS in the case of T7. We are going to extract by PCR the RNA polymerase of the phage T3 and clone it under the control of a promoter inducible with IPTG. In both cases we expect to see the precense of fluorescence under uv light.
Quorum sensing system
After assembling the device, we will turn on the system by any of the RNA polymerases. We expect to see green florescence in the point of induction and red in the neigborhood.
asRNA
We will induce the asRNA construction with IPTG, afterwards we expect the infection of T7 and T3 to be with less efficience of plaquing.
Toxins
The colicines will be under the transcriptional regulation of the promoter induced by IPTG. We will messure the optical density of the culture just as performed avobe. We will provide the results to the model. We expect the growth curve of this strain to decay before than that of the infection with phages.
