Team:Uppsala-Sweden/Ethanol

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The Pathway

Light energy is used in the Calvin cycle to transform water and carbon dioxide into organic compounds, among those is Ribulose-1,5-bisphosphate, which immediately splits into two 3-Phosphoglycerate molecules. [2] These two molecules can now be converted to 2-Phosphoglycerate. Enolase and pyruvatekinase finally catalyze then the reaction over phosphoenolpyruvate to pyruvate. [3] This pyruvate molecule is now substrate for the ethanol production. A nice overview be found further down.

blabal mechanism

References

[1] [http://www.ncbi.nlm.nih.gov/pubmed/9925577 Ethanol synthesis by genetic engineering in cyanobacteria. Deng MD, Coleman JR. Appl Environ Microbiol. 1999 Feb;65(2):523-8]

[2] Jeremy M. Berg, John L. Tymoczko, L. Stryer Biochemistry 5th Edition; Ch.21.1 p826-838

[3] Jeremy M. Berg, John L. Tymoczko, L. Stryer Biochemistry 5th Edition; Ch.16.1 p653

[4] [http://biocyc.org/META/NEW-IMAGE?type=REACTION&object=RXN-7643 MetaCyc Reaction: 4.1.1.1]

[5] [http://biocyc.org/META/NEW-IMAGE?type=REACTION&object=RXN-7657 MetaCyc Reaction: 1.1.1.1]

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 ==The Pathway==
-The idea to produce ethanol with the use of cyanobacteria was originally proposed and already accomplished by Ming-De Deng and John R. Coleman. [[#ref1|<nowiki>[1]</nowiki>]] We from the Uppsala iGEM Team decided to try to improve the production of ethanol by interfering with the metabolic pathways of <i>Synechocystis sp. PCC 6803</i> by an [https://2009.igem.org/Team:Uppsala-Sweden/Ethanol Antisense RNA] and a [https://2009.igem.org/Team:Uppsala-Sweden/rna protein-mediated] approach. Hence we build a construct that encodes for ethanol production in our host organism. But first of all the some theory about the molecular mechanism.
+Light energy is used in the Calvin cycle to transform water and carbon dioxide into organic compounds, among those is Ribulose-1,5-bisphosphate, which immediately splits into two 3-Phosphoglycerate molecules. [[#ref2|<nowiki>[2]</nowiki>]] These two molecules can now be converted to 2-Phosphoglycerate. Enolase and pyruvatekinase finally catalyze then the reaction over phosphoenolpyruvate to pyruvate. [[#ref3|<nowiki>[3]</nowiki>]] This pyruvate molecule is now substrate for the ethanol production. A nice overview be found further [[#refview|<nowiki>down</nowiki>]].
 <center>
 [[Image:ethanolpath_horizontal.png]]
 </center>
-Light energy is used in the Calvin cycle to transform water and carbon dioxide into organic compounds, among those is Ribulose-1,5-bisphosphate, which immediately splits into two 3-Phosphoglycerate molecules. [[#ref2|<nowiki>[2]</nowiki>]] These two molecules can now be converted to 2-Phosphoglycerate. Enolase and pyruvatekinase finally catalyze then the reaction over phosphoenolpyruvate to pyruvate. [[#ref3|<nowiki>[3]</nowiki>]] This pyruvate molecule is now substrate for the ethanol production. A nice overview be found further [[#refview|<nowiki>down</nowiki>]].
 blabal mechanism