Team:LCG-UNAM-Mexico/Resources/Strains

(Difference between revisions)

Revision as of 00:25, 22 October 2009

C-1a.- Main strain for working with phage P2 and P4. This is also the mother strain for all the modified phage-containing derivatives. Its genetic map is really close to E. coli K12 "pop" strain. Its genome is being sequenced.

C-331 /arg/-am/1/ /tonA-trp/D, str-r (P2) (P4 /imp/). The abbreviation "imp" means "improved plaques."

C-1895 F^+ /supD60/ (P2 /lg/), which we use to grow and sometimes assay for P4. The symbol "lg" denotes large plaques.

C-2423 /trp/-am /arg/-am/1/ str-r (P2 /lg/ del/1/). This strain is used to get the best P4 plaques. The symbol del1 denotes a

deletion of the /old /gene, which interferes with the growth of phage lambda and kills /recB-/ bacteria.

C-1895 sp+.- this slow-growing strain can be used for obtaining an excelent stock of P4 sid1

- BL21(DE3)pLysS: widely used for protein expression deficient in lon and ompT proteases. It has a chromosomal copy of the T7 RNA polymerase and T7 lysozyme for suppression o basal expression of T7 RNA polymerase and avoid as much as possible

cell growth and viability affection.

TERRIBLY, UNCOUNTABLE, PERSISTENT and ANTIBIOTIC RESISTANT!!!

Retrieved from "http://2009.igem.org/Team:LCG-UNAM-Mexico/Resources/Strains"

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 ===Expression Strains===
-BL21(DE3)pLysS: widely used for protein expression deficient in lon and ompT proteases. It has a chromosomal copy of the T7 RNA polymerase and T7 lysozyme for suppression o basal expression of T7 RNA polymerase and avoid as much as possible
+**BL21(DE3)pLysS: widely used for protein expression deficient in lon and ompT proteases. It has a chromosomal copy of the T7 RNA polymerase and T7 lysozyme for suppression o basal expression of T7 RNA polymerase and avoid as much as possible
 cell growth and viability affection.