Team:LCG-UNAM-Mexico/Wet Lab/Objectives
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====Fernando Montaño==== | ====Fernando Montaño==== | ||
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- | **Assembly of the P4 vector | + | **Assembly of the [http://partsregistry.org/wiki/index.php?title=Part:BBa_K242051 P4 vector] |
We want P4 to work as a [[Team:LCG-UNAM-Mexico/Description#Delivery|transduction vector for biobricks]]. Several things need to be done, which include <br>individual amplification of P4 sid1 essential region, which we expect to be sufficient for a stable permanence <br>of the biobrick inside the cell. To test this, the first biobrick added will be a reporter gene, which is intended <br>to permanently stay with our P4 version. This is enough to further produce our P4 viral particles and assess<br> functionality of the delivery by transduction into several wild-type bacterial strains as reported in literature. | We want P4 to work as a [[Team:LCG-UNAM-Mexico/Description#Delivery|transduction vector for biobricks]]. Several things need to be done, which include <br>individual amplification of P4 sid1 essential region, which we expect to be sufficient for a stable permanence <br>of the biobrick inside the cell. To test this, the first biobrick added will be a reporter gene, which is intended <br>to permanently stay with our P4 version. This is enough to further produce our P4 viral particles and assess<br> functionality of the delivery by transduction into several wild-type bacterial strains as reported in literature. |
Revision as of 23:00, 21 October 2009