Team:LCG-UNAM-Mexico/Wet Lab/Experiments
From 2009.igem.org
(Difference between revisions)
(→asRNA) |
(→Multipromotor) |
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presented a diminished florescence. | presented a diminished florescence. | ||
- | This result was only for T7 RNA polymerase but we are planning to implement a better assay system to include T3 | + | This result was only for T7 RNA polymerase but we are planning to implement a better assay system to include a WT T3 & T7 |
- | polymerase | + | RNA polymerase, a plasmid that have GFP and not the multipromoter and a strain that doesn't have T7 RNA polymerase. Also |
+ | we need to clone the biocrick in a plasmid that only has our multipromoter and no other in the same direction this to avoid licking from near upstream promoters, this is important beacuse we are using quite toxic bacterial proteins | ||
+ | that will be induced by this promoter when T3 or T7 phages and it is very important to estimate the net basal transcription. | ||
====Quorum sensing system==== | ====Quorum sensing system==== |
Revision as of 23:40, 21 October 2009