Revision as of 11:02, 8 July 2009

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Project task-management notebook

Week 1 - BioBricks, primers, protocols and more

Plasmid backbone

Task: Find a backbone for both e.coli and b. subtilis Status: Doing. People: Helle and Mike Last edit: July 8th 2009.

July 7th 2009: We found the backbone, [http://partsregistry.org/wiki/index.php/Part:BBa_I742123 BBa_I742123], which other teams have found to be compatible with both both gram positive and gram negative bacteria, such as e. coli.

After talking to HQ, it turns out that the quality of their stock is bad, and they will try to get some home from older teams. Furthermore, it might simply be better to use a more specifik backbone for e. coli and for b. subtilis.

(mike)

July 8th 2009: We decided to start working with [http://partsregistry.org/Part:pSB1A3 pSB1A3] instead, which is a high-output e. coli backbone.

Output: 100-300 number pr. cell. Resistance: ampicillin (we're going with 50 µg/mL first)

(mike)

Inducible promoter

7.July Anna

I found the inducible promoter BBa_R0011 that is regulated by LacI.

It is a strong promoter, that will be on in stains without LacI.

E. coli strain

The plasmid seems to be working in most types (no need for ccdB) Jacob suggests E. coli KG22, which can turns on the operon by adding IPTG.

@@ Line 9: / Line 9: @@
-=Project management notebook=
+=Project task-management notebook=
 ==Week 1 - BioBricks, primers, protocols and more ==
@@ Line 15: / Line 15: @@
 ===Plasmid backbone===
-.july: Helle and Mike
+'''Task:''' Find a backbone for both e.coli and b. subtilis
+'''Status:''' Doing.
+'''People:''' Helle and Mike
+'''Last edit:''' July 8th 2009.
-We found a plasmid backbone, that works in both e. coli and b. subtilis (that is, both in gram negative and gram positive bacteria). It has a quality star.
+''July 7th 2009:''
+We found the backbone, [http://partsregistry.org/wiki/index.php/Part:BBa_I742123 BBa_I742123], which other teams have found to be compatible with both both gram positive and gram negative bacteria, such as e. coli.
-The plasmid backbone is called BBa_I742123, and can be found here: http://partsregistry.org/wiki/index.php/Part:BBa_I742123
+After talking to HQ, it turns out that the quality of their stock is bad, and they will try to get some home from older teams. Furthermore, it might simply be better to use a more specifik backbone for e. coli and for b. subtilis.
-It seems that the backbone works with all e. coli type, both we're not a 100%.
+(mike)
-The backbone is resistant to the following antibiotics: chloramphenicol, neomycin & gentamicin.
+''July 8th 2009:''
+We decided to start working with [http://partsregistry.org/Part:pSB1A3 pSB1A3] instead, which is a high-output e. coli backbone.
+Output: 100-300 number pr. cell.
+Resistance: ampicillin (we're going with 50 µg/mL first)
+(mike)
-Ordering: we've written HQ, requesting the part since we cant figuar out how to get it via the registry.
 ===Inducible promoter===

Team:SDU-Denmark/Notebook

From 2009.igem.org