Team:UAB-Barcelona/PCRP

From 2009.igem.org

(Difference between revisions)

Revision as of 02:16, 22 October 2009

Documento sin título

PCR of nitrite detection sequences

We used GoTaq Green Master Mix (Promega)1 2

Annealing temperature

phoA (100 pb): as annealing temperature is 74ºC, the temperature we should set in the thermociclator would be 69ºC. But it didn't work properly at this temperature, so we set the temperature to 66ºC and 64ºC. PCR in both cases worked properly

lambda-DNA hinIII; 3 x phoa 64ºC; control without primers; control without DNA template; lambda-DNA hinIII; 3 x phoa 66ºC; control without primers; control without DNA template; lambda-DNA hinIII

Revision as of 23:55, 21 October 2009 (view source) Antonio.freire (Talk \| contribs) (→PCR of nitrite detection sequences) ← Older edit		Revision as of 02:16, 22 October 2009 (view source) Antonio.freire (Talk \| contribs) (→PCR of nitrite detection sequences) Newer edit →
Line 10:		Line 10:

	https://static.igem.org/mediawiki/2009/3/37/PhoA.jpg		https://static.igem.org/mediawiki/2009/3/37/PhoA.jpg
		+
		+	lambda-DNA hinIII; 3 x phoa 64ºC; control without primers; control without DNA template; lambda-DNA hinIII; 3 x phoa 66ºC; control without primers; control without DNA template; lambda-DNA hinIII