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Team:BIOTEC Dresden/Notebook SilverNano
From 2009.igem.org
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| - | [1] Lee, S., et al., ''Science'', 296, 892-895 <br> | + | [1] Lee, S., et al., ''Science'', 296, 892-895, 2002 <br> |
[2] Naik, R., et al., ''Nat. Materials'', 1, 169-172, 2002 <br> | [2] Naik, R., et al., ''Nat. Materials'', 1, 169-172, 2002 <br> | ||
[3] Naik, R., et al., ''Nanobiophot. Biomed. Appl.'', 5331, 1605-7422, 2004 | [3] Naik, R., et al., ''Nanobiophot. Biomed. Appl.'', 5331, 1605-7422, 2004 | ||
Revision as of 00:41, 22 October 2009
Silver Nano Particles
Strategy
Unique peptides have been found capable of nucleating metal atoms, spurred by an interest in biomineralization by the prospect of nanoscale building [1]. We use a twelve amino-acid long silver binding peptide that has been identified by combinatorial screening [2,3] to nucleate silver nanoparticles. The peptide is tagged with GFP so that it can be monitored along the flow chamber. Several protocols to bio-synthesize silver nanoparticles were tried in parallel, and absorbance of various samples were measured using spectrophotometry.
Here the basic ideas are introduced.
Lab notes and obtained results.
[1] Lee, S., et al., Science, 296, 892-895, 2002
[2] Naik, R., et al., Nat. Materials, 1, 169-172, 2002
[3] Naik, R., et al., Nanobiophot. Biomed. Appl., 5331, 1605-7422, 2004
