Team:Harvard/Protocols

From 2009.igem.org

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<br> Bacteria-yeast communication <br>
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<br>  
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      <a href="https://2009.igem.org/Team:Harvard/Bacteria-Yeast Communication">Bacteria Yeast Communication</a>
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        <br>
<a href="http://74.125.93.132/search?q=cache:nsdCILcgZcQJ:www.stratagene.com/manuals/200518.pdf+stratagene+site+directed+mutagensis&cd=1&hl=en&ct=clnk&gl=us&client=firefox-a">Site directed mutagenesis</a> <br>
<a href="http://74.125.93.132/search?q=cache:nsdCILcgZcQJ:www.stratagene.com/manuals/200518.pdf+stratagene+site+directed+mutagensis&cd=1&hl=en&ct=clnk&gl=us&client=firefox-a">Site directed mutagenesis</a> <br>
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<a href="http://www.neb.com/nebecomm/products/protocol64.asp>PCR amplification</a><br>
<a href="http://www.neb.com/nebecomm/products/protocol64.asp>PCR amplification</a><br>
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Regular Ligation
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    <ul>
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      <a href="https://2009.igem.org/Team:Harvard/Regular Ligation">Regular Ligation</a>
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    </ul>
<a href="http://www.neb.com/nebecomm/products/protocol2.asp"> Quick ligation </a><br>
<a href="http://www.neb.com/nebecomm/products/protocol2.asp"> Quick ligation </a><br>
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Restriction digest<br>
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     <ul>
     <ul>
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       <li><a href="https://2009.igem.org/Team:Harvard/Restriction Digest">Restriction Digest</a></li>
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       <a href="https://2009.igem.org/Team:Harvard/Restriction Digest">Restriction Digest</a>
     </ul>
     </ul>
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Colony PCR<br>
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      <a href="https://2009.igem.org/Team:Harvard/Colony PCR">Colony PCR</a>
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    <br>
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Growing cultures<br>
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      <a href="https://2009.igem.org/Team:Harvard/Growing Cultures">Growing Cultures</a>
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    <br>
  <a href="http://74.125.93.132/search?q=cache:D60LJ_yepbYJ:kirschner.med.harvard.edu/files/protocols/QIAGEN_QIAprepMiniprepKit_EN.pdf+quiagen+miniprep&cd=2&hl=en&ct=clnk&gl=us&client=firefox-a"> Miniprep</a> <br><a href="http://docs.google.com/gview?a=v&q=cache:YBOZok5AVokJ:www.virology.wisc.edu/acp/Plasmids/PlasmidFiles/Protocols.pdf+quiagen+maxiprep&hl=en&gl=us&pid=bl&srcid=ADGEESj2Vc0xg_muu5BVON57ZJq_ZMX667-nK3iHjoL4JDKHUhBAniGHFMLMUtV5MjrlJb3rtdJ4AC8GAruJ-PeiawAUFupobPi8s1Fs4MjqpPknqKvj3OGrUH0Whadl4uYMGuymYpsu&sig=AFQjCNHvA6VFrdNYLWD9D11U-XJsDAiLQQ">Maxiprep</a>
  <a href="http://74.125.93.132/search?q=cache:D60LJ_yepbYJ:kirschner.med.harvard.edu/files/protocols/QIAGEN_QIAprepMiniprepKit_EN.pdf+quiagen+miniprep&cd=2&hl=en&ct=clnk&gl=us&client=firefox-a"> Miniprep</a> <br><a href="http://docs.google.com/gview?a=v&q=cache:YBOZok5AVokJ:www.virology.wisc.edu/acp/Plasmids/PlasmidFiles/Protocols.pdf+quiagen+maxiprep&hl=en&gl=us&pid=bl&srcid=ADGEESj2Vc0xg_muu5BVON57ZJq_ZMX667-nK3iHjoL4JDKHUhBAniGHFMLMUtV5MjrlJb3rtdJ4AC8GAruJ-PeiawAUFupobPi8s1Fs4MjqpPknqKvj3OGrUH0Whadl4uYMGuymYpsu&sig=AFQjCNHvA6VFrdNYLWD9D11U-XJsDAiLQQ">Maxiprep</a>

Revision as of 02:41, 22 October 2009

Hi Mom

Protocols

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Bacteria Yeast Communication
Site directed mutagenesis
Regular Ligation Quick ligation

Colony PCR
Growing Cultures
Miniprep
Maxiprep
PCR purification
Gel purification
Darkroom construction

Team

Projects

Parts

References

Lab Notebook

Ethics