Team:IPN-UNAM-Mexico/Parts

From 2009.igem.org

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| [[Image:Control1.jpg | 250px|center]] || Regulatory LacI module coupled to the activator module with GFP reporter module. Basal conditions. 6,4x
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Regulatory LacI module coupled to the activator module with GFP reporter module. Basal conditions. 19x
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Regulatory LacI module coupled to the activator module with GFP reporter module. IPTG 160mM. 19x
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Revision as of 03:09, 22 October 2009


BannerUNAM.jpg

Biobricks

Month-icon.pngBiobricks

We contribute to the registry with 11 new biobricks, 5 of them are favorite biobricks from the team and in this section we document them:

Main biobricks: modules and Projects

Modules

Module and Status Biobrick Name Type Image Description
Mod 1 Sent [http://partsregistry.org/Part:BBa_K266006 BBa_K266006] Las AHL
Las AHL
 BBa_K091146 promoter (PAI+LasR inducible & AI+LuxR repressible) controls the policistronic expresion of LasI enzyme and GFP, double terminator.
Mod 2 Sent [http://partsregistry.org/Part:BBa_K266004 BBa_K266004] Lac inverter
Lac Inverter
 Constitutive promoter J23100 with Lac system inverter controlling the expression of LasR.
Mod 3 Sent [http://partsregistry.org/Part:BBa_K266001 BBa_K266001] Tet inverter
AI Tet.jpg
 J23100 constitutive promoter directs the expression of tetracycline repressor (TetR). TetR binds to pTet regulatory region resulting in a negative control of the production of LuxR. The whole system acts as an Inverter of Tet system controlling LuxR expression. TetR repression is inhibited by the addition of tetracycline or its analog, aTc.
Mod 4 Sent [http://partsregistry.org/Part:BBa_K266000 BBa_K266000] Lux AHL
Lux AHL
 This biobrick has a promoter inducible by PAI+LasR (BBa_R0079) I.e. positive regulation and produces LuxI enzyme (BBa_F1610). This enzyme produces 3OC6HSL (AI).


Projects

These two biobricks are the main Activator-Inhibitor system, as showned below they are in two different plasmids, and the idea is transform Top10 cells with them in petri dishes with IPTG and ATC.

Status Biobrick Name Image Description
Sent [http://partsregistry.org/Part:BBa_K266007 BBa_K266007]
substrates
 Complex Quorum sensing circuit that receives the signal of PAI+LasR and AI+LuxR to control the production of LuxI and LasI enzymes.
Pending [http://partsregistry.org/Part:BBa_K266010 BBa_K2660010]
substrates
 Tet constitutive inverter controlling LasR expression and Lac constitutive inverter controlling LuxR expression.

Auxiliary biobricks

This biobricks are though like construction intermediates necessary to build the main and project biobricks.

Status Biobrick Name Image Description
Sent [http://partsregistry.org/Part:BBa_K266002 BBa_K266002]
substrates
 LasR coding region and a double terminator.
Sent [http://partsregistry.org/Part:BBa_K266005 BBa_K266005]
substrates
 BBa_K091146 promoter (PAI+LasR inducible & AI+LuxR repressible) controls the expresion of LasI enzyme, no terminator.
Sent [http://partsregistry.org/Part:BBa_K266008 BBa_K266008]
substrates
 The BBa_J23100 constitutive promoter with Lac systems inverter.
Sent [http://partsregistry.org/Part:BBa_K266009 BBa_K266009]
substrates
 The BBa_J23100 constitutive promoter with Lac systems inverter and strong RBS.
Sent [http://partsregistry.org/Part:BBa_K266011 BBa_K2660011]
substrates
 POPS regulated and Lac inverter system with strong RBS.

Month-icon.pngPlasmids

Check registry for more info with the name of the biobrick AI Biobricks.jpg] AI Biobricks2.jpg AI Biobricks3.jpg AI Biobricks4.jpg AI Biobricks5.jpg AI Biobricks6.jpg AI Biobricks7.jpg AI Biobricks8.jpg AI Biobricks9.jpg AI Biobricks10.jpg AI Biobricks11.jpg AI Biobricks12.jpg

Month-icon.pngResults

Control1.jpg
 Regulatory LacI module coupled to the activator module with GFP reporter module. Basal conditions. 6,4x
1601.jpg
 Regulatory LacI module coupled to the activator module with GFP reporter module. IPTG 160mM. 6,4x
Control2.jpg
 Regulatory LacI module coupled to the activator module with GFP reporter module. Basal conditions. 19x
1602.jpg
 Regulatory LacI module coupled to the activator module with GFP reporter module. IPTG 160mM. 19x

Banner footer UNAM2.jpg

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