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Team:Todai-Tokyo/Notebook/bioclock
From 2009.igem.org
(Difference between revisions)
| Line 3: | Line 3: | ||
'''Methods:'''<BR> | '''Methods:'''<BR> | ||
| - | + | 1. Clone the following genes from ''Bacillus subtilis'' into a biobrick vector.<BR> | |
#plate1,7D<BR> | #plate1,7D<BR> | ||
| Line 10: | Line 10: | ||
#plate2,1H<BR> | #plate2,1H<BR> | ||
| - | + | 2. Make a gene network that express oscillatory pattern, using these genes.<BR> | |
= Cloning the parts= | = Cloning the parts= | ||
Revision as of 07:48, 9 July 2009
Plan
Aim: Create E.coli that show oscillatory gene expression pattern
Methods:
1. Clone the following genes from Bacillus subtilis into a biobrick vector.
- plate1,7D
- plate1,13B
- plate1,13L
- plate2,1H
2. Make a gene network that express oscillatory pattern, using these genes.
Cloning the parts
7/7
preculture of following Biobrick parts for Miniprep
7/8
Miniprep of E.coli cells containing LDLR gene with Promega, Wizard Plus SV Miniprep DNA Purification System
failure
7/9
Miniprep again of E.coli cells containing LDLR gene with Promega, Wizard Plus SV Miniprep DNA Purification System
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