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Virginia Commonwealth/9 July 2009
From 2009.igem.org
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(New page: ==Thursday 9 July 2009== ===Results=== ''Afton and Maria'' *Electrotransformation was successful **Control (+) NEB 10 beta on LB has lawn growth **Control (+) NEB 10 beta on LB electroshoc...) |
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* Make a glycerol stock to go in the -80 degree Celsius freezer | * Make a glycerol stock to go in the -80 degree Celsius freezer | ||
[[User:Trentay|Trentay]] 14:06, 9 July 2009 (UTC) | [[User:Trentay|Trentay]] 14:06, 9 July 2009 (UTC) | ||
| + | ''Kevin and Adam'' | ||
| + | * Back bone pSB3K3 is a bad part. Cultivate backbone pSB1C3 from glycerol stock. | ||
| + | * Proceed with analytical work and ordering DNA. | ||
---- | ---- | ||
====Wetlab==== | ====Wetlab==== | ||
Revision as of 14:40, 9 July 2009
Contents |
Thursday 9 July 2009
Results
Afton and Maria
- Electrotransformation was successful
- Control (+) NEB 10 beta on LB has lawn growth
- Control (+) NEB 10 beta on LB electroshocked has lawn growth
- Control (-) NEB 10 beta on AMP has no growth
- J23106 (promoter) on AMP has hundreds of little colonies
- J06702 (RET) on AMP has about one hundred colonies
Trentay 14:06, 9 July 2009 (UTC)
Tasks
Afton and Maria
- Pick cells for overnight culture
- Make a glycerol stock to go in the -80 degree Celsius freezer
Trentay 14:06, 9 July 2009 (UTC) Kevin and Adam
- Back bone pSB3K3 is a bad part. Cultivate backbone pSB1C3 from glycerol stock.
- Proceed with analytical work and ordering DNA.
Wetlab
Afton and Maria
- 1
- 2
Trentay 14:06, 9 July 2009 (UTC)
