Team:IBB Pune/project/project3

From 2009.igem.org

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Project 3 is concerned with developing a system which is naturally competent. This is to be done by introducing ''com'' genes taken from naturally competent strains of ''Acinetobacter'' and ''Streptococcus'' into a suitable vector imparting natural competence and readiness of the bacterium to acquire genes. Induction of competence will thus enable the bacteria to take up naked DNA from its surroundings which will greatly facilitate transformation experiments.
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Project 3 is so far the last idea, which is concerned with developing a system which is naturally competent. This is effected by introducing ''com'' genes taken from naturally competent strains of ''Acinetobacter'' and ''Streptococcus'' into a suitable vector imparting natural competence and readiness of the bacterium to acquire genes. Induction of competence will thus enable the bacteria to take up naked DNA from its surroundings which will greatly facilitate transformation experiments.
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This has several applications.
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We also plan to introduce a lytic cassette which, when introduced into a subpopulation of cells will induce their lysis and also subsequently spread to the rest of the population thereby giving rise to a chain reaction of lysis events, creating a 'programmed lysis'in the particular sub-population, although the temporal evaluation of these genes remains the toughest challenge to us.
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This has a wide range of important applications in
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1) Preparation of DNA,plasmid
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2) Extraction of protein
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3) Clinical applications like in DNA vaccines etc.
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4) Therapeutic applications in case of pathogenic bacteria
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[[Image:picture1.png|600px|thumb|center|alt text]]]
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Revision as of 20:00, 10 July 2009

Project 3 is concerned with developing a system which is naturally competent. This is to be done by introducing com genes taken from naturally competent strains of Acinetobacter and Streptococcus into a suitable vector imparting natural competence and readiness of the bacterium to acquire genes. Induction of competence will thus enable the bacteria to take up naked DNA from its surroundings which will greatly facilitate transformation experiments.

This has several applications.

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