Team:UNC Chapel Hill/Project Needs

From 2009.igem.org

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# PCR Supermix
# PCR Supermix
# Competent Cells (DH10B, Top10, or DH5a.  DB3.1)
# Competent Cells (DH10B, Top10, or DH5a.  DB3.1)
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# Power Generators for GE
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# Gel Rigs
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# Gel Combs
==Equipment==
==Equipment==

Revision as of 22:04, 10 July 2009

Basic Lab Supplies

  1. Blank Petri Dishes
  2. Electric cuvettes
  3. MiniPrep Kit
  4. Disposable Gloves (S, M, L)
  5. Screw Top Microcentrifuge Tubes (1.5 mL)
  6. Cell Culture Tubes (10 mL)
  7. Eppendorf Tubes (1.5 mL)
  8. Glass Pasteur pipets (9 mL)
  9. Parafilm
  10. Micropipets (P2, P20, P200, P1000)
  11. Micropipet Tips (for all sizes listed above)
  12. Restriction Enzymes-if from Fermentas, should be FastDigest versions. If NEB, we need EcoRI-HF:
    1. EcoRI
    2. Xbal
    3. SpeI
    4. PstI
    5. MunI (MfeI)
    6. Mph1103I (NsiI)
  13. T4 DNA Ligase
  14. Agarose Powder
  15. Luria Broth and/or Super Optimal Broth
  16. Buffer for Gel Electrophoresis
  17. Antibiotics:
    1. Carbenicillin
    2. Kanamycin
  18. Gel Extraction Kit
  19. PCR Supermix
  20. Competent Cells (DH10B, Top10, or DH5a. DB3.1)
  21. Power Generators for GE
  22. Gel Rigs
  23. Gel Combs

Equipment

  1. Thermal Cycler
  2. Fluorometer

Other

  1. Plane tickets to Boston for Jamboree weekend (October 31 to November 2nd, 2009)
  2. Hotel stay in Boston for Jamboree
  3. Attendance fee for Jamboree ($175 per student; $375 per advisor)
  4. Custom T-shirts