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Team:EPF-Lausanne
From 2009.igem.org
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'''Keep track with what we did so far''' | '''Keep track with what we did so far''' | ||
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:This first week of wetlab we have done the following things | :This first week of wetlab we have done the following things | ||
*Transformed the plasmids with the LovTAP gene, generously sent by Dr. Sosnick's lab from Chicago universtiy, into competent E.Coli | *Transformed the plasmids with the LovTAP gene, generously sent by Dr. Sosnick's lab from Chicago universtiy, into competent E.Coli | ||
Revision as of 15:45, 12 July 2009
Contents |
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Last News
Keep track with what we did so far
(12.07.09)
- This first week of wetlab we have done the following things
- Transformed the plasmids with the LovTAP gene, generously sent by Dr. Sosnick's lab from Chicago universtiy, into competent E.Coli
- Designed the cloning strategy for cloning the LovTAP gene from its original vector to a iGEM vector+add an incducible promoter (LacI) (+RBS +term.)
- Orderd and received the primers needed for the PCR of LovTAP
- Designed the cloning strategy for inclusion of the LovTAP BioBrick with different reporting cassettes
- Transformed all the BioBricks that will be needed for the cloning strategies (c.f. notebook for more information about this parts) into competent E.Coli
- Fused the two BioBricks LacI and term
- Digested the LovTAP PCR product and RBS part
