(→Tuesday, July 14, 2009) |
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| Line 32: | Line 32: | ||
3) #2 OmpC | 3) #2 OmpC | ||
| - | 4) #2 Destination plasmid | + | 4) #2 Destination plasmid (pSB1K3) |
| - | 5) #2 Dest. plasmid | + | 5) #2 Dest. plasmid (pSB1K3) |
6) #2 GFP | 6) #2 GFP | ||
-Colony PCR of cells transformed with previous ligations
-PCR to add KpnI and BamHI sites to GFP
-Spectrophotometry of dbcOmega strains in ATCC media
-PCR to add BioBrick prefix and suffix to Cph8 and pRKCBC3
-Transformed S17 competent cells to check efficiency. OD = 0.115
-Performed Triligation and Silver Lab Ligations to fuse OmpC+GFP
-Started cultures of dbcOmega in LB+Strep in lieu of ATCC
Gel #1: Silver
1) Ladder
2) #1 OmpC
3) #1 OmpC
4) #1 GFP
5) #1 GFP
6) Ladder
Gel #2: BioBrick
1) Ladder
2) #2 OmpC
3) #2 OmpC
4) #2 Destination plasmid (pSB1K3)
5) #2 Dest. plasmid (pSB1K3)
6) #2 GFP
7) #2 GFP
8) Ladder
|
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