Team:Wash U/Calendar-Home/14 July 2009

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Revision as of 21:55, 14 July 2009

Tuesday, July 14, 2009

-Colony PCR of cells transformed with previous ligations
-PCR to add KpnI and BamHI sites to GFP
-Spectrophotometry of dbcOmega strains in ATCC media
-PCR to add BioBrick prefix and suffix to Cph8 and pRKCBC3
-Transformed S17 competent cells to check efficiency. OD = 0.115
-Performed Triligation and Silver Lab Ligations to fuse OmpC+GFP
-Started cultures of dbcOmega in LB+Strep in lieu of ATCC

Gel #1: Silver digestion protocol

1) Ladder

2) OmpC + pSB1A2 plasmid (2187 bp)

3) OmpC + pSB1A2 plasmid

4) GFP (876 bp)

5) GFP

6) Ladder

Gel #2: BioBrick (Triligation) digestion protocol

1) Ladder

2) OmpC (108 bp)

3) OmpC

4) Destination plasmid, pSB1K3 (2206 bp)

5) Dest. plasmid, pSB1K3

6) GFP (876 bp)

7) GFP

8) Ladder

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@@ Line 14: / Line 14: @@
 ) Ladder
-) OmpC (108 bp)
+) OmpC + pSB1A2 plasmid (2187 bp)
-) OmpC
+) OmpC + pSB1A2 plasmid
 ) GFP (876 bp)
@@ Line 28: / Line 28: @@
 ) Ladder
-) OmpC
+) OmpC (108 bp)
 ) OmpC
-) Destination plasmid (pSB1K3)
+) Destination plasmid, pSB1K3 (2206 bp)
-) Dest. plasmid (pSB1K3)
+) Dest. plasmid, pSB1K3
-) GFP
+) GFP (876 bp)
 ) GFP