Team:Wash U/Protocol
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# [[Team:Wash_U/Protocol#Genomic DNA Purification|Genomic DNA Purification]] | # [[Team:Wash_U/Protocol#Genomic DNA Purification|Genomic DNA Purification]] | ||
# [[Team:Wash_U/Protocol#Gel Extraction|Gel Extraction]] | # [[Team:Wash_U/Protocol#Gel Extraction|Gel Extraction]] | ||
+ | # [[Team:Wash_U/Protocol#Making Competent Cells|Making Competent Cells]] | ||
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# Add 700uL of Wash Solution to the column and cetrifuge for 1 minute and discard the flow through. Centrifuge the column again for 1 minute to remove excess ethanol. | # Add 700uL of Wash Solution to the column and cetrifuge for 1 minute and discard the flow through. Centrifuge the column again for 1 minute to remove excess ethanol. | ||
# Transfer the column to a new collection tube. Add 50uL of preheated Elution solution (65C) to the column and centrifuge for 1 minute. The desired DNA is now in the collection tube. | # Transfer the column to a new collection tube. Add 50uL of preheated Elution solution (65C) to the column and centrifuge for 1 minute. The desired DNA is now in the collection tube. | ||
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+ | =='''Making Competent Cells'''== | ||
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Revision as of 21:17, 15 July 2009