Team:Wash U/Protocol
From 2009.igem.org
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* 2.5 microliters EtBr (Caution: EtBr is a known carcinogen) | * 2.5 microliters EtBr (Caution: EtBr is a known carcinogen) | ||
* Note: Jacob suggested adding 1.0 microliter EtBr to gel and 3.0 microliters to TAE buffer in rig. | * Note: Jacob suggested adding 1.0 microliter EtBr to gel and 3.0 microliters to TAE buffer in rig. | ||
+ | '''CCMB80 Buffer''' | ||
+ | * 10 mM KOAc pH 7.0 (10 ml of a 1M stock/L) | ||
+ | * 80 mM CaCl2.2H2O (11.8 g/L) | ||
+ | * 20 mM MnCl2.4H2O (4.0 g/L) | ||
+ | * 10 mM MgCl2.6H2O (2.0 g/L) | ||
+ | * 10% glycerol (100 ml/L) | ||
+ | * adjust pH DOWN to 6.4 with 0.1N HCl if necessary <br> | ||
+ | '''Note''':adjusting pH up will precipitate manganese dioxide from Mn containing solutions. | ||
+ | * sterile filter and store at 4°C | ||
+ | * slight dark precipitate appears not to affect its function | ||
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[https://2009.igem.org/Team:Wash_U/Protocol Back To Top] | [https://2009.igem.org/Team:Wash_U/Protocol Back To Top] | ||
{{WashUbottom}} | {{WashUbottom}} |
Revision as of 21:22, 15 July 2009