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Virginia Commonwealth/9 July 2009
From 2009.igem.org
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| - | * The three parts for limonene synthesis ( | + | * The three parts for limonene synthesis (BBa_I742111, BBa_K118024, and BBa_K118025) will be electrotransformed today. They will also be plated and grown overnight on ampicillin-selective media. |
* Tomorrow colonies will be selected and grown in ampicillin-selective liquid media during the day. Later in the evening a miniprep will be performed on the grown parts. | * Tomorrow colonies will be selected and grown in ampicillin-selective liquid media during the day. Later in the evening a miniprep will be performed on the grown parts. | ||
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Revision as of 17:04, 17 July 2009
Contents |
Thursday 9 July 2009
Results
Afton and Maria
- Electrotransformation was successful
- Control (+) NEB 10 beta on LB has lawn growth
- Control (+) NEB 10 beta on LB electroshocked has lawn growth
- Control (-) NEB 10 beta on AMP has no growth
- J23106 (promoter) on AMP has hundreds of little colonies
- J06702 (RET) on AMP has about one hundred colonies
Trentay 14:06, 9 July 2009 (UTC)
Tasks
Afton and Maria
- Pick cells for overnight culture
Trentay 19:45, 9 July 2009 (UTC)
Kevin and Adam
- Proceed with analytical work and ordering DNA.
Craig and Clay
- The three parts for limonene synthesis (BBa_I742111, BBa_K118024, and BBa_K118025) will be electrotransformed today. They will also be plated and grown overnight on ampicillin-selective media.
- Tomorrow colonies will be selected and grown in ampicillin-selective liquid media during the day. Later in the evening a miniprep will be performed on the grown parts.
Wetlab
Afton and Maria
- Put 5 colonies of J23106 in 5mL of LB (two test tube)
- Put 5 colonies of J06702 in 5mL of LB (one test tube)
Trentay 21:13, 9 July 2009 (UTC)
Craig and Clay
- The following parts for limonene synthesis were electrotransformed into the NEB 10 beta strain of E coli: BBa_I742111, BBa_K118024, and BBa_K118025. All DNA was obtained from the distribution plates. These electrotransformed parts were then plated on ampicillin-selective plates and left to grow overnight.
