Virginia Commonwealth/22 July 2009

(Difference between revisions)

Revision as of 20:03, 22 July 2009

Overnight cultures were successful
- Vials had noticeable amounts of white E. coli at the bottom of the tube.
Flow Cytometer was repaired
- Samples cannot be tested until protocols for using and maintaining the machine are figured out
Plated bacteria are still not expressing GFP and RFP

Dilute E. coli into fresh LB + Cm in preparation for testing with the flow cytometer
- nothing was tested today with the cytometer
Grow all BioBrick parts in 5 mL of LB + Cm overnight
- 100 microliters was added to broth
- plastic tubes had tops, so tops were loosened during the incubation period
- cells were out of the -80 degree Celsius freezer for a prolonged amount of time, so viability is lower than usual.
  - If nothing grows, BioBrick DNA will be reinserted into fresh NEB 10 beta through electrotransformation.
Retransform J23100 w/ J06702
Transform NEB 10 beta and DB 3.1 to check for electrocompetency
Organize notebook and running draft

Trentay 19:57, 22 July 2009 (UTC)

@@ Line 16: / Line 16: @@
 ** cells were out of the -80 degree Celsius freezer for a prolonged amount of time, so viability is lower than usual.
 *** If nothing grows, BioBrick DNA will be reinserted into fresh NEB 10 beta through electrotransformation.
+* Retransform J23100 w/ J06702
+* Transform NEB 10 beta and DB 3.1 to check for electrocompetency
 * Organize notebook and running draft
 [[User:Trentay|Trentay]] 19:57, 22 July 2009 (UTC)