Virginia Commonwealth/23 July 2009

From 2009.igem.org

(Difference between revisions)
(New page: ''Maria and Afton'' * Samples from cryogenic stocks were grown up in LB: ** DB 3.1 (2 vials) * Samples from cryogenic stocks were grown up in LB + Amp: ** pSB4C5 * Samples from cryogenic s...)
Line 1: Line 1:
 +
=Thursday 23 July 2009==
 +
===Results===
''Maria and Afton''
''Maria and Afton''
-
* Samples from cryogenic stocks were grown up in LB:
+
* All overnight cultures grew except one vial of NEB 10 beta seed stock
-
** DB 3.1 (2 vials)
+
** there was a second vial of seed stock that did grow successfully
-
* Samples from cryogenic stocks were grown up in LB + Amp:
+
* Transformations were successful
-
** pSB4C5
+
-
* Samples from cryogenic stocks were grown up in LB + Cm:
+
-
{| cellpadding="10" align="center" border="0.9" padding="5"
+
-
|+
+
-
|-
+
-
! Promoter !! RET !! Growth Observation !!
+
-
|-
+
-
! J23100
+
-
|E0240|| n/a ||
+
-
|-
+
-
! J23101
+
-
|E0240||    ||
+
-
|-
+
-
! J23101 
+
-
|J06702||    ||
+
-
|-
+
-
! J23102
+
-
|E0240||  ||
+
-
|-
+
-
! J23102
+
-
|J06702||    ||
+
-
|-
+
-
! J23103
+
-
|E0240||  ||
+
-
|-
+
-
! J23103
+
-
|J06702||    ||
+
-
|-
+
-
! J23104
+
-
|E0240||    ||
+
-
|-
+
-
! J23104
+
-
|J06702||    ||
+
-
|-
+
-
! J23105
+
-
|E0240||    ||
+
-
|-
+
-
! J23105
+
-
|J06702||    ||
+
-
|-
+
-
! J23106
+
-
|E0240||    ||
+
-
|-
+
-
! J23106
+
-
|J06702||    ||
+
-
|-
+
-
! J23107
+
-
|E0240||    ||
+
-
|-
+
-
! J23107
+
-
|J06702||    ||
+
-
|-
+
-
! J23110
+
-
|E0240||    ||
+
-
|-
+
-
! J23110
+
-
|J06702||    ||
+
-
|-
+
-
! J23113
+
-
|E0240||    ||
+
-
|-
+
-
! J23113
+
-
|J06702||    ||
+
-
|-
+
-
! J23116
+
-
|E0240||    ||
+
-
|-
+
-
! J23116
+
-
|J06702||    ||
+
-
|-
+
-
! R0011
+
-
|E0240||    ||
+
-
|-
+
-
! R0011
+
-
|J06702||    ||
+
-
|-
+
-
! R0040
+
-
|E0240||    ||
+
-
|-
+
-
! R0040
+
-
|J06702||    ||
+
-
|-
+
-
|}
+
-
* Transformation of electrocompetent E. Coli stock and BioBrick with promoter J23100 and RET J06702
+
{| cellpadding="10" align="center" border="0.9" padding="5"
{| cellpadding="10" align="center" border="0.9" padding="5"
|+  
|+  
Line 113: Line 31:
| electro comp. || Cm || J23100 w/ J06702 || 6 small colonies ||
| electro comp. || Cm || J23100 w/ J06702 || 6 small colonies ||
|}
|}
-
[[User:Trentay|Trentay]] 14:18, 23 July 2009 (UTC)
+
 
 +
----
 +
===Tasks===
 +
''Maria and Afton''
 +
*
 +
----
 +
====Wetlab====
 +
''Maria and Afton''
 +
* D

Revision as of 16:52, 23 July 2009

Contents

Thursday 23 July 2009=

Results

Maria and Afton

  • All overnight cultures grew except one vial of NEB 10 beta seed stock
    • there was a second vial of seed stock that did grow successfully
  • Transformations were successful
Name Type Plate Purpose Growth Observation
NEB 10 beta seed stock LB make more seed stock lawn
NEB 10 beta electro comp. LB (+) Control/ make more stock lawn
NEB 10 beta electro comp. LB (+) Control shocked lawn
DB 3.1 electro comp. LB (+) Control shocked lawn
DB 3.1 electro comp. LB (+) Control/make more stock lawn
NEB 10 beta electro comp. Cm (-) Control no growth
NEB 10 beta electro comp. Cm J23100 w/ J06702 6 small colonies

Tasks

Maria and Afton

Wetlab

Maria and Afton

  • D
Retrieved from "http://2009.igem.org/Virginia_Commonwealth/23_July_2009"