Virginia Commonwealth/20 July 2009

From 2009.igem.org

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|align="center" width="150pt"|{{#calendar: title=Virginia_Commonwealth |year=2009 | month=10}}
 
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==Monday 20 July 2009==
 
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===Results===
 
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* Results of transformation with pSB4C5 backbone in NEB 10 beta three days after transformation
 
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** Control (+) on LB NEB 10 beta (not shocked) had Lawn growth
 
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** Control (-) on Cm NEB 10 beta (not shocked) had No growth
 
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{| cellpadding="10" align="center" border="0.9" padding="5"
 
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|+
 
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|-
 
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! Promoter!! Reporter !! Resistance/Plate !! Growth Observation !!
 
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|-
 
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! J23110
 
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| E0240 || Cm || 62 colonies ||
 
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|-
 
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! J23110
 
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| J06702 || Cm || 56 colonies ||
 
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|-
 
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! J23113
 
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| E0240 || Cm || 75 colonies ||
 
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|-
 
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! J23113
 
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| J06702 || Cm || 33 colonies ||
 
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|-
 
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! J23116
 
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| E0240 || Cm || 41 colonies ||
 
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|-
 
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! J23116
 
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| J06702 || Cm || 65 colonies ||
 
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|-
 
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! R0011
 
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| E0240 || Cm || hundreds of colonies ||
 
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|-
 
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! R0011
 
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| J06702 || Cm || 5 colonies ||
 
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|-
 
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! R0040
 
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| E0240 || Cm || 20 colonies ||
 
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|-
 
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! R0040
 
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| J06702 || Cm || 12 colonies ||
 
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|-
 
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! J23105
 
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| E0240 || Cm || 26 colonies ||
 
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|-
 
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! J23101
 
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| E0240 || Cm || 34 colonies ||
 
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|}
 
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[[User:Trentay|Trentay]] 14:17, 20 July 2009 (UTC)
 
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''Craig and Clay''
 
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* The registry did confirm that the correct resistance for the limonene synthesis related parts is ampicillin.  New parts will also be sent.
 
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----
 
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===Tasks===
 
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''Maria and Afton''
 
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* Pick colonies for overnight culture from transformations
 
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* Ligate J23106 and J23101 with backbone pSB4C5 and RET E0240
 
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* Transform liagated parts into NEB 10 beta
 
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[[User:Trentay|Trentay]] 18:40, 20 July 2009 (UTC)
 
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----
 
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====Wetlab====
 
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*1
 
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*2
 

Revision as of 14:31, 24 July 2009