Team:MoWestern Davidson/project wetlab
From 2009.igem.org
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We began our project design by constructing 12 suppressor tRNAs by oligo assembly. Suppressor tRNA genes were ligated with the pBad promoter to allow weak constitutive and inducible tRNA expression. | We began our project design by constructing 12 suppressor tRNAs by oligo assembly. Suppressor tRNA genes were ligated with the pBad promoter to allow weak constitutive and inducible tRNA expression. | ||
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+ | [[Image:Pbad trna.JPG]] |