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Minnesota/22 June 2009
From 2009.igem.org
(Difference between revisions)
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|'''[[Minnesota/19 June 2009|Go to Previous Day (June 19)]]'''|| width=158|'''[[Minnesota/23 June 2009|Go to Next Day (June 23)]]''' | |'''[[Minnesota/19 June 2009|Go to Previous Day (June 19)]]'''|| width=158|'''[[Minnesota/23 June 2009|Go to Next Day (June 23)]]''' | ||
| + | |} | ||
| + | Here is the first incarnation of the model (many future models will simply change this one): | ||
| + | {| class="wikitable" style="text-align:center" border = "1" | ||
| + | |- | ||
| + | ! Reaction | ||
| + | ! Forward Kinetic Constant | ||
| + | ! Reverse Kinetic Constant | ||
| + | |- | ||
| + | | RNAp + lacP + lacI4:lacO1 -> RNAp:lacP||6.23E+05|| | ||
| + | |- | ||
| + | | RNAp + lacP + lacO1 ↔ RNAp:lacP||1E+07|| 1 | ||
| + | |- | ||
| + | |RNA:lacP -> RNAp:lacP* || .01 || | ||
| + | |- | ||
| + | |RNAp:lacP* -> lacP + lacO1 + RNAp:DNAgfp||30|| | ||
| + | |- | ||
| + | |RNAp:DNAgfp -> RNAp + gfp_mRNA||30|| | ||
| + | |- | ||
| + | |gfp_mRNA + rib -> rib:gfp_mRNA||100000|| | ||
| + | |- | ||
| + | |rib:gfp_mRNA -> rib:gfp_mRNA_1 + gfp_mRNA||33|| | ||
| + | |- | ||
| + | |rib:gfp_mRNA_1 -> rib + gfp||33|| | ||
| + | |- | ||
| + | |tetR2 + aTc ↔ tetR2:aTc||2E+09||4E-04 | ||
| + | |- | ||
| + | |tetR2:aTc + aTc ↔ tetR2:aTc2||1E+08||1E-03 | ||
| + | |- | ||
| + | |tetR2:aTc + tetO1 ↔ tetR2:tetO1:aTc||1E+08||1 | ||
| + | |- | ||
| + | |tetR2:aTc2 + tetO1 ↔ tetR2:tetO1:aTc2||1E+08||1E+05 | ||
| + | |- | ||
| + | |tetR2:tetO1 + aTc ↔ tetR2:tetO1:aTc||1E+08||1E-03 | ||
| + | |- | ||
| + | |tetR2:tetO1:aTc + aTc ↔ tetR2:tetO1:aTc2||1E+08||1E-03 | ||
| + | |- | ||
| + | |tetR2 -> Ø||2.89E-04|| | ||
| + | |- | ||
| + | |tetR2:aTc -> aTc||2.89E-04|| | ||
| + | |- | ||
| + | |tetR2:aTc2 -> 2 aTc||2.89E-04|| | ||
| + | |- | ||
| + | |tetR2 + nsDNA ↔ tetR2:nsDNA||1000||3.2409 | ||
| + | |- | ||
| + | |tetR2:aTc + nsDNA ↔ tetR2:aTc:nsDNA||1000||3.2409 | ||
| + | |- | ||
| + | |tetR2:aTc:nsDNA -> aTc + nsDNA||1.93E-04|| | ||
| + | |- | ||
| + | |tetR2:nsDNA -> nsDNA||1.93E-04|| | ||
| + | |- | ||
| + | |Ø -> tetR2||1E-11|| | ||
| + | |- | ||
| + | |Ø -> aTc||3.3E-04|| | ||
| + | |- | ||
| + | |gfp_mRNA -> Ø||1.16E-03|| | ||
| + | |- | ||
| + | |gfp -> Ø||3.21E-05|| | ||
|} | |} | ||
Revision as of 15:37, 29 July 2009
| Back to Notebook Home | |
| Go to Previous Day (June 19) | Go to Next Day (June 23) |
Here is the first incarnation of the model (many future models will simply change this one):
| Reaction | Forward Kinetic Constant | Reverse Kinetic Constant |
|---|---|---|
| RNAp + lacP + lacI4:lacO1 -> RNAp:lacP | 6.23E+05 | |
| RNAp + lacP + lacO1 ↔ RNAp:lacP | 1E+07 | 1 |
| RNA:lacP -> RNAp:lacP* | .01 | |
| RNAp:lacP* -> lacP + lacO1 + RNAp:DNAgfp | 30 | |
| RNAp:DNAgfp -> RNAp + gfp_mRNA | 30 | |
| gfp_mRNA + rib -> rib:gfp_mRNA | 100000 | |
| rib:gfp_mRNA -> rib:gfp_mRNA_1 + gfp_mRNA | 33 | |
| rib:gfp_mRNA_1 -> rib + gfp | 33 | |
| tetR2 + aTc ↔ tetR2:aTc | 2E+09 | 4E-04 |
| tetR2:aTc + aTc ↔ tetR2:aTc2 | 1E+08 | 1E-03 |
| tetR2:aTc + tetO1 ↔ tetR2:tetO1:aTc | 1E+08 | 1 |
| tetR2:aTc2 + tetO1 ↔ tetR2:tetO1:aTc2 | 1E+08 | 1E+05 |
| tetR2:tetO1 + aTc ↔ tetR2:tetO1:aTc | 1E+08 | 1E-03 |
| tetR2:tetO1:aTc + aTc ↔ tetR2:tetO1:aTc2 | 1E+08 | 1E-03 |
| tetR2 -> Ø | 2.89E-04 | |
| tetR2:aTc -> aTc | 2.89E-04 | |
| tetR2:aTc2 -> 2 aTc | 2.89E-04 | |
| tetR2 + nsDNA ↔ tetR2:nsDNA | 1000 | 3.2409 |
| tetR2:aTc + nsDNA ↔ tetR2:aTc:nsDNA | 1000 | 3.2409 |
| tetR2:aTc:nsDNA -> aTc + nsDNA | 1.93E-04 | |
| tetR2:nsDNA -> nsDNA | 1.93E-04 | |
| Ø -> tetR2 | 1E-11 | |
| Ø -> aTc | 3.3E-04 | |
| gfp_mRNA -> Ø | 1.16E-03 | |
| gfp -> Ø | 3.21E-05 |
