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Team:Groningen/Parts
From 2009.igem.org
(Difference between revisions)
Svenjurgens (Talk | contribs) (→Part List -80) |
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| Line 24: | Line 24: | ||
|'''Resistance''' | |'''Resistance''' | ||
|'''Well''' | |'''Well''' | ||
| + | |'''Quality control''' | ||
|- | |- | ||
|GVP | |GVP | ||
| Line 32: | Line 33: | ||
|Ampicillin/Gentamycin | |Ampicillin/Gentamycin | ||
|plate 3, pos.1 | |plate 3, pos.1 | ||
| + | |? | ||
|- | |- | ||
|GVP (2nd batch) | |GVP (2nd batch) | ||
| Line 40: | Line 42: | ||
|Ampicillin/Gentamycin | |Ampicillin/Gentamycin | ||
|plate 3, pos.2 | |plate 3, pos.2 | ||
| + | |? | ||
|- | |- | ||
| - | | | + | |pHigh in low copy nr vector |
|High consitutive promoter | |High consitutive promoter | ||
|<partinfo>J23100</partinfo> | |<partinfo>J23100</partinfo> | ||
| Line 48: | Line 51: | ||
|Kanamycin | |Kanamycin | ||
|Plate 3, pos. 4 | |Plate 3, pos. 4 | ||
| + | |PCR(?), restriction(+) | ||
|- | |- | ||
| - | | | + | |pMed in low copy nr vector |
|Medium consitutive promoter | |Medium consitutive promoter | ||
|<partinfo>J23106</partinfo> | |<partinfo>J23106</partinfo> | ||
| Line 56: | Line 60: | ||
|Kanamycin | |Kanamycin | ||
|Plate 3, pos. 5 | |Plate 3, pos. 5 | ||
| + | |PCR(?), restriction(+) | ||
|- | |- | ||
| - | | | + | |pLow in low copy nr vector |
|Low consitutive promoter | |Low consitutive promoter | ||
|<partinfo>J23109</partinfo> | |<partinfo>J23109</partinfo> | ||
| Line 64: | Line 69: | ||
|Kanamycin | |Kanamycin | ||
|Plate 3, pos. 6 | |Plate 3, pos. 6 | ||
| + | |PCR(?), restriction(+) | ||
|- | |- | ||
| - | | | + | |pHigh in high copy nr vector |
|High consitutive promoter | |High consitutive promoter | ||
|<partinfo>J23100</partinfo> | |<partinfo>J23100</partinfo> | ||
| Line 72: | Line 78: | ||
|Ampicillin/Chloramphenicol | |Ampicillin/Chloramphenicol | ||
|Plate 3, pos. 7 | |Plate 3, pos. 7 | ||
| + | |PCR(?), restriction(+) | ||
|- | |- | ||
| - | | | + | |pMed in high copy nr vector |
|Medium consitutive promoter | |Medium consitutive promoter | ||
|<partinfo>J23106</partinfo> | |<partinfo>J23106</partinfo> | ||
| Line 80: | Line 87: | ||
|Ampicillin/Chloramphenicol | |Ampicillin/Chloramphenicol | ||
|Plate 3, pos. 8 | |Plate 3, pos. 8 | ||
| + | |PCR(?), restriction(+) | ||
|- | |- | ||
| - | | | + | |pLow in high copy nr vector |
|High consitutive promoter | |High consitutive promoter | ||
|<partinfo>J23109</partinfo> | |<partinfo>J23109</partinfo> | ||
| Line 88: | Line 96: | ||
|Ampicillin/Chloramphenicol | |Ampicillin/Chloramphenicol | ||
|Plate 3, pos. 9 | |Plate 3, pos. 9 | ||
| + | |PCR(?), restriction(+) | ||
|- | |- | ||
|Promotor (low expression) | |Promotor (low expression) | ||
| Line 96: | Line 105: | ||
|Ampicillin | |Ampicillin | ||
|plate 3, pos.10 | |plate 3, pos.10 | ||
| + | |No | ||
|- | |- | ||
|Promotor (medium expression) | |Promotor (medium expression) | ||
| Line 104: | Line 114: | ||
|Ampicillin | |Ampicillin | ||
|plate 3, pos.11 | |plate 3, pos.11 | ||
| + | |No | ||
|- | |- | ||
|Promotor (high expression) | |Promotor (high expression) | ||
| Line 112: | Line 123: | ||
|Ampicillin | |Ampicillin | ||
|plate 3, pos.12 | |plate 3, pos.12 | ||
| + | |No | ||
|- | |- | ||
|Inducible promoter | |Inducible promoter | ||
| Line 120: | Line 132: | ||
|Ampicillin | |Ampicillin | ||
|Plate 3, pos. 14 | |Plate 3, pos. 14 | ||
| + | |PCR(?), restriction(+) | ||
|- | |- | ||
|Inducible promoter | |Inducible promoter | ||
| Line 128: | Line 141: | ||
|Ampicillin | |Ampicillin | ||
|Plate 3, pos. 15 | |Plate 3, pos. 15 | ||
| + | |PCR(?), restriction(+) | ||
|- | |- | ||
|RBS | |RBS | ||
| Line 136: | Line 150: | ||
|Ampicillin | |Ampicillin | ||
|plate 3, pos.19 | |plate 3, pos.19 | ||
| + | |No | ||
|- | |- | ||
|Terminator | |Terminator | ||
| Line 144: | Line 159: | ||
|Ampicillin/Kanamycin | |Ampicillin/Kanamycin | ||
|plate 3, pos.20 | |plate 3, pos.20 | ||
| + | |No | ||
|- | |- | ||
|HmtA (1.1) | |HmtA (1.1) | ||
| - | | | + | |HmtA-His in pBAD(a) |
|None | |None | ||
|? | |? | ||
| Line 152: | Line 168: | ||
|Ampicillin | |Ampicillin | ||
|plate 3, pos.28 | |plate 3, pos.28 | ||
| + | |Restriction(+) | ||
|- | |- | ||
|HmtA (2.1) | |HmtA (2.1) | ||
| - | | | + | |HmtA-His in pBAD(a) |
|None | |None | ||
|? | |? | ||
| Line 160: | Line 177: | ||
|Ampicillin | |Ampicillin | ||
|plate 3, pos.29 | |plate 3, pos.29 | ||
| + | |Restriction(+) | ||
|} | |} | ||
Revision as of 13:50, 4 August 2009
[http://2009.igem.org/Team:Groningen http://2009.igem.org/wiki/images/f/f1/Igemhomelogo.png]
| Biobricks
|
|---|
Note
If you choose to include a Parts Submitted to the Registry page, please list your parts here. This is not necessary but it may be a nice list to keep track of.
- http://partsregistry.org/wiki/images/d/db/Buttonaddpart.gif
[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=Groningen Our sandbox]
Part List -80
| Component | Description | Part or Accession # | Base Pairs (bp) | Plasmid (backbone) | Resistance | Well | Quality control |
| GVP | Gas Vesicle Proteins | [http://partsregistry.org/Part:BBa_I750016 BBa_I750016] | 6064 | [http://partsregistry.org/Part:BBa_J61035 BBa_J61035] | Ampicillin/Gentamycin | plate 3, pos.1 | ? |
| GVP (2nd batch) | Gas Vesicle Proteins | [http://partsregistry.org/Part:BBa_I750016 BBa_I750016] | 6064 | [http://partsregistry.org/Part:BBa_J61035 BBa_J61035] | Ampicillin/Gentamycin | plate 3, pos.2 | ? |
| pHigh in low copy nr vector | High consitutive promoter | 35bp | Kanamycin | Plate 3, pos. 4 | PCR(?), restriction(+) | ||
| pMed in low copy nr vector | Medium consitutive promoter | 35bp | Kanamycin | Plate 3, pos. 5 | PCR(?), restriction(+) | ||
| pLow in low copy nr vector | Low consitutive promoter | 35bp | Kanamycin | Plate 3, pos. 6 | PCR(?), restriction(+) | ||
| pHigh in high copy nr vector | High consitutive promoter | 35bp | Ampicillin/Chloramphenicol | Plate 3, pos. 7 | PCR(?), restriction(+) | ||
| pMed in high copy nr vector | Medium consitutive promoter | 35bp | Ampicillin/Chloramphenicol | Plate 3, pos. 8 | PCR(?), restriction(+) | ||
| pLow in high copy nr vector | High consitutive promoter | 35bp | Ampicillin/Chloramphenicol | Plate 3, pos. 9 | PCR(?), restriction(+) | ||
| Promotor (low expression) | Constitutive promotor | [http://partsregistry.org/Part:BBa_J23109 BBa_J23109] | 35 | [http://partsregistry.org/Part:BBa_J61002 BBa_J61002] | Ampicillin | plate 3, pos.10 | No |
| Promotor (medium expression) | Constitutive promotor | [http://partsregistry.org/Part:BBa_J23106 BBa_J23106] | 35 | [http://partsregistry.org/Part:BBa_J61002 BBa_J61002] | Ampicillin | plate 3, pos.11 | No |
| Promotor (high expression) | Constitutive promotor | [http://partsregistry.org/Part:BBa_J23100 BBa_J23100] | 35 | [http://partsregistry.org/Part:BBa_J61002 BBa_J61002] | Ampicillin | plate 3, pos.12 | No |
| Inducible promoter | Lactose inducible promoter nr1 | 200bp | Ampicillin | Plate 3, pos. 14 | PCR(?), restriction(+) | ||
| Inducible promoter | Lactose inducible promoter nr2 | 200bp | Ampicillin | Plate 3, pos. 15 | PCR(?), restriction(+) | ||
| RBS | Ribosomal Binding Site | [http://partsregistry.org/Part:BBa_B0034 BBa_B0034] | 12 | [http://partsregistry.org/Part:pSB1A2 pSB1A2] | Ampicillin | plate 3, pos.19 | No |
| Terminator | Double terminator consisting of [http://partsregistry.org/Part:BBa_B0012 BBa_B0012] and [http://partsregistry.org/Part:BBa_B0011 BBa_B0011] | [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] | ~90 | [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] | Ampicillin/Kanamycin | plate 3, pos.20 | No |
| HmtA (1.1) | HmtA-His in pBAD(a) | None | ? | None | Ampicillin | plate 3, pos.28 | Restriction(+) |
| HmtA (2.1) | HmtA-His in pBAD(a) | None | ? | None | Ampicillin | plate 3, pos.29 | Restriction(+) |
