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Team:Bologna
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[[Image:TeamBologna.jpg|frame|center|Our Team]] | [[Image:TeamBologna.jpg|frame|center|Our Team]] | ||
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| + | = Project Description = | ||
| - | + | '''Which is our idea?''' | |
| + | Our project aims the realization of a device for gene expression regulation at translation level, regardless of the target gene. | ||
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| - | + | '''How can we achieve this?''' | |
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| - | + | We designed a 50bp non coding sequence (CIS - repressing) to be assembled upstream of the target gene RBS, while its complementary sequence (TRANS - repressor) is to be placed under the control of another promoter. The TRANS - repressing contains also a cover, that is a short sequence complementary to the RBS. | |
| - | + | When the TRANS-repressor element is present, it binds to the CIS-repressing, forming a RNA duplex and producing an obstruction that prevents the ribosome binding to the RBS. | |
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== Calendar == | == Calendar == | ||
Revision as of 09:28, 31 July 2009
| HOME | PROJECT | TEAM | SOFTWARE | MODELING | WETLAB | LAB-BOOK | SUBMITTED PARTS | BIOSAFETY AND PROTOCOLS |
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Our Team
Project Description
Which is our idea?
Our project aims the realization of a device for gene expression regulation at translation level, regardless of the target gene.
How can we achieve this?
We designed a 50bp non coding sequence (CIS - repressing) to be assembled upstream of the target gene RBS, while its complementary sequence (TRANS - repressor) is to be placed under the control of another promoter. The TRANS - repressing contains also a cover, that is a short sequence complementary to the RBS. When the TRANS-repressor element is present, it binds to the CIS-repressing, forming a RNA duplex and producing an obstruction that prevents the ribosome binding to the RBS.
