Team:KULeuven/Freezer
From 2009.igem.org
(Difference between revisions)
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= Blue light receptor = | = Blue light receptor = | ||
- | {| class="Generic" | + | {| class="Generic sortable" |
! class='top' | part | ! class='top' | part | ||
! class='top' | plasmid | ! class='top' | plasmid | ||
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= Key/antikey/lock = | = Key/antikey/lock = | ||
- | {| class="Generic" | + | {| class="Generic sortable" |
! class='top' | part | ! class='top' | part | ||
! class='top' | plasmid | ! class='top' | plasmid | ||
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= vanillin synthesis = | = vanillin synthesis = | ||
- | {| class="Generic" | + | {| class="Generic sortable" |
! class='top' | part | ! class='top' | part | ||
! class='top' | plasmid | ! class='top' | plasmid | ||
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= vanillin receptor = | = vanillin receptor = | ||
- | {| class="Generic" | + | {| class="Generic sortable" |
! class='top' | part | ! class='top' | part | ||
! class='top' | plasmid | ! class='top' | plasmid | ||
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= miscellaneous = | = miscellaneous = | ||
- | {| class="Generic" | + | {| class="Generic sortable" |
! class='top' | part | ! class='top' | part | ||
! class='top' | plasmid | ! class='top' | plasmid |
Revision as of 11:21, 31 July 2009
Contents |
Blue light receptor
part | plasmid | description | status | μl left | concentration | date |
---|---|---|---|---|---|---|
MC4100 | / | strain of E.coli | july 24 | |||
2171 | / | forward primer for | 90µl | 20μM | july 24 | |
2172 | / | reverse primer for | 90µl | 20μM | july 24 | |
2171 | / | forward primer for | 100μM | july 24 | ||
2172 | / | reverse primer for | 100μM | july 24 | ||
pSB1A2 | GFP + rbs + terminator | purified | july 29 | |||
pSB1A2 | GFP + rbs + terminator | restricted with EcoRI and XbaI | july 29 | |||
/ | BLR promoter region | restricted with EcoRI | july 29 | |||
/ | BLR promoter region | 4 microcentrifuge tubes | july 30 |
Key/antikey/lock
part | plasmid | description | status | μl left | concentration | date |
---|---|---|---|---|---|---|
/ | GFP with LVA tag | purified | july 28 | |||
/ | tetR promoter | purified | july 28 |
vanillin synthesis
part | plasmid | description | status | μl left | concentration | date |
---|---|---|---|---|---|---|
pSB1A2 | ech + rbs | put in competent cells | july 28 | |||
pSB1A2 | fcs + rbs | put in competent cells | july 28 |
vanillin receptor
part | plasmid | description | status | μl left | concentration | date |
---|
miscellaneous
part | plasmid | description | status | μl left | concentration | date |
---|---|---|---|---|---|---|
dNTP mix | ATP, GTP, TTP, CTP | 94 | 10μM | July 24 | ||
pSB1A2 | medium constitutieve promoter | July 23 | ||||
ampicilline |