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Team:Bologna/Project
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!align="center"|[[Team:Bologna/Software|<font color="#ffffff">SOFTWARE</font>]] | !align="center"|[[Team:Bologna/Software|<font color="#ffffff">SOFTWARE</font>]] | ||
!align="center"|[[Team:Bologna/Modeling|<font color="#ffffff">MODELING</font>]] | !align="center"|[[Team:Bologna/Modeling|<font color="#ffffff">MODELING</font>]] | ||
| - | + | !align="center"|[[Team:Bologna/Notebook|<font color="#ffffff">LAB-NOTEBOOK</font>]] | |
| - | !align="center"|[[Team:Bologna/Notebook|<font color="#ffffff">LAB- | + | |
!align="center"|[[Team:Bologna/Parts|<font color="#ffffff">SUBMITTED PARTS</font>]] | !align="center"|[[Team:Bologna/Parts|<font color="#ffffff">SUBMITTED PARTS</font>]] | ||
| - | !align="center"|[[Team:Bologna/Biosafety|<font color="#ffffff"> | + | !align="center"|[[Team:Bologna/Biosafety|<font color="#ffffff">HUMAN PRACTICE</font>]] |
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== '''Introduction''' == | == '''Introduction''' == | ||
Revision as of 13:22, 31 July 2009
| HOME | PROJECT | TEAM | SOFTWARE | MODELING | LAB-NOTEBOOK | SUBMITTED PARTS | HUMAN PRACTICE |
|---|
Introduction
Our goal is to create a logic gate based on a post-transcriptional regulation system in Escherichia coli, using RNA to silence gene expression. We inserted a cis-acting sequence directly upstream of the rbs (ribosome binding site) to realize a "regulated rbs". We also designed the complementary trans-repressing sequence whose function is to recognize and cover the "regulated rbs" and prevent translation from it. Two versions of the trans- repressing sequence were designed with 2 different kinds of rbs covers. We want to use this short non-coding RNA segment placed in trans, to silence translation from dowstream the cis-acting sequence. We developed a bioinformatic tool to research the sequences and these are the results:
| Cis-repressing sequence, inserted upstream of the target gene | |||
| Scar | Cis-repressing | Rbs | Scar |
| TACTAGAG | AACACAAACTATCACTTTAACAACACATTACATATACATTAAAATATTAC | AAAGAGGAGAAA | TACTAGAG |
| Trans-repressor sequence with a cover of 7 bases (long version) | |||
| Scar | Cover | Trans(long) | Scar |
| TACTAGAG | CCTCTTT | GTAATATTTTAATGTATATGTAATGTGTTGTTAAAGTGATAGTTTGTGTT | TACTAGAG |
| Trans-repressor sequence with a cover of 4 bases (short version) | |||
| Scar | Cover | Trans(short) | Scar |
| TACTAGAG | CTTT | GTAATATTTTAATGTATATGTAATGTGTTGTTAAAGTGATAGTTTGTGTT | TACTAGAG |
The sequence secondary structures of RNAs are obtained using the RNA mfold tool:
These are the three genetic circuits that we are currently studying:
