Team:DTU Denmark

From 2009.igem.org

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<h1><BLINK>Page under construction! Stay tuned for bio-engineering awesomeness!</BLINK></h1>
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<title>Welcome to the DTU iGEM wiki!</title>
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    <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2009.igem.org/Team:DTU_Denmark" >Home</a></font> </td>
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    <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2009.igem.org/Team:DTU_Denmark/team" >The Team</a> </font></td>
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    <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2009.igem.org/Team:DTU_Denmark/project" >The Project</a> </font></td>
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    <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2009.igem.org/Team:DTU_Denmark/parts" >Parts submitted</a> </font></td>
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    <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2009.igem.org/Team:DTU_Denmark/modelling">Modelling</a></font> </td>
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    <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2009.igem.org/Team:DTU_Denmark/notebook" title="Day to day lab activity">Notebook</a>
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    I wonder what menu we will need here?<br><br>
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    <a href="http://www.gaffa.dk" CLASS=leftbar>- Gaffa.dk</a><br>
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    <br>
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    <br>And what about here?<br><br>
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    <a href="http://www.gaffa.dk" CLASS=leftbar>- Gaffa.dk</a><br>
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  <b>DTU denmark</b><br><br>
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<font size="3"><b>The Redoxilator</b></font><br>
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By in silico design and computer modelling followed by gene synthesis, we have constructed a molecular NAD/NADH ratio sensing system in Saccharomyces cerevisiae. The sensor works as an inducible transcription factor being active only at certain levels of the NAD/NADH ratios. By the coupling of a yeast optimized fast degradable GFP, the system can be used for in vivo monitoring of NAD/NADH redox poise. A future novel application of the system is heterologous redox coupled protein production in yeast.
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<font size="3"><b>The USER fusion standard</b></font><br>
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Another part of our project is the proposal of a new parts-assembly standard for Biobricks based on USER(TradeMark) cloning. With this technique, not based on restriction enzymes, all parts independent of function can be assembled without leaving any scars from the restriction enzyme digestions.
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  <b>Medal status</b><br><br>
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We surely have the gold by now!
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Comments or questions to the team? Please <a href="mailto:igem@bio.dtu.dk" CLASS=email>Email us</a> -- Comments of questions to webmaster? Please <a href="mailto:lronn@bio.dtu.dk" CLASS=email>Email us</a>
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<h2>Project description:</h2>
 
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<h3>The Redoxilator</h3>
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The aim of this project is to model and construct a molecular NAD<sup>+</sup>/NADH ratio sensing system in Saccharomyces cerevisiae by a synthetic biology approach. The sensor will work as an inducible transcription factor, being active only at certain levels of the NAD<sup>+</sup>/NADH ratios. By coupling the sensor to the expression of a yeast optimized fast degradable GFP, the system can be used for monitoring the NAD<sup>+</sup>/NADH redox poise in vivo. Other applications of the system could be heterologous redox coupled protein production in yeast.
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<h3>The USER fusion standard</h3>
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Another part of our project is the proposal of a new parts-assembly standard for Biobricks based on USER<sup>TM</sup> cloning. With this technique, not based on restriction enzymes, all parts independent of function can be assembled without leaving any ‘scars’ from the restriction enzyme digestions. Furthermore we will provide a software for easing the process of designing primers for the purpose of USER fusion.
 
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Revision as of 15:29, 23 September 2009

Wiki banner 967px.png

Welcome to the DTU iGEM wiki!

Home The Team The Project Parts submitted Modelling Notebook
I wonder what menu we will need here?

 - Gaffa.dk
And what about here?

 - Gaffa.dk
 		DTU denmark

 The Redoxilator
By in silico design and computer modelling followed by gene synthesis, we have constructed a molecular NAD/NADH ratio sensing system in Saccharomyces cerevisiae. The sensor works as an inducible transcription factor being active only at certain levels of the NAD/NADH ratios. By the coupling of a yeast optimized fast degradable GFP, the system can be used for in vivo monitoring of NAD/NADH redox poise. A future novel application of the system is heterologous redox coupled protein production in yeast.

The USER fusion standard
Another part of our project is the proposal of a new parts-assembly standard for Biobricks based on USER(TradeMark) cloning. With this technique, not based on restriction enzymes, all parts independent of function can be assembled without leaving any scars from the restriction enzyme digestions. 		Medal status

We surely have the gold by now!
Comments or questions to the team? Please -- Comments of questions to webmaster? Please

Retrieved from "http://2009.igem.org/Team:DTU_Denmark"