Ann's Notebook

From 2009.igem.org

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*Digestion of the RBS for Pu promoter and the Bacteriophage lysis cassette
*Digestion of the RBS for Pu promoter and the Bacteriophage lysis cassette
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[[media:7_25_09_Nano_Drop_of_RBS_and_Killer_genes_and_Digest_with_EcoRI_and_XbaI_of_RBS_and_Bactreriophage_lysis_cassette.pdf| 7/25/09]]
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[[media:7_27_09_Gel_of_RBS_and_Bacteriophage_lysis_cassette_digest_Plates_for_Pu_promoter_colony_PCR_Streaking_out_Pu_luciferase_part_from_the_registry.pdf| 7/27/09]]

Revision as of 23:23, 3 August 2009

7/22/09 and 7/23/09

  • Media preparation for transformation
  • Comp. cell protocol
  • Protocol for getting parts from the biobricks
  • Transformation protocol
  • Transformation of RBS for Pu promoter, Bacteriophage lysis cassette, lambda lysis device, T4 lysis device

7/24/09

  • Protocol for miniprep
  • Miniprep of RBS for Pu promoter, Bacteriophage lysis cassette, lambda lysis device, T4 lysis device

7/25/09

  • Protocol for using the nanodrop
  • Protocol for a digestion
  • Nanodrop concentrations of the RBS for Pu promoter, Bacteriophage lysis cassette, lambda lysis device, T4 lysis device
  • Digestion of the RBS for Pu promoter and the Bacteriophage lysis cassette

7/27/09