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Modelling
From 2009.igem.org
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<br>- Model allosteric interactions between LOVTAP & TrpR | <br>- Model allosteric interactions between LOVTAP & TrpR | ||
<br>- Do a mutational assay to change specificity of LOVTAP: | <br>- Do a mutational assay to change specificity of LOVTAP: | ||
| - | Direct mutational assay: Insert mutation in specific sites thanks to the known structure of LOVTAP (already modeled on computer) | + | <br> Direct mutational assay: Insert mutation in specific sites thanks to the known structure of LOVTAP (already modeled on computer) |
| + | <br> Indirect mutational assay: Random mutations, then selection of the "right" protein according to a set of selected conditions | ||
<br>- Model the fusion of LOV domain with other transcription factors | <br>- Model the fusion of LOV domain with other transcription factors | ||
Revision as of 17:58, 26 May 2009
To find
PDB files of LOV domain, TrpR repressor => http://www.rcsb.org/pdb/home/home.do
To do
- Set up day of starting modeling, do a planning for modeling => see with Matteo Dal Peraro
- Model allosteric interactions between LOVTAP & TrpR
- Do a mutational assay to change specificity of LOVTAP:
Direct mutational assay: Insert mutation in specific sites thanks to the known structure of LOVTAP (already modeled on computer)
Indirect mutational assay: Random mutations, then selection of the "right" protein according to a set of selected conditions
- Model the fusion of LOV domain with other transcription factors
To envisage
- Molecular mutationnal assay
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