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LOVTAP
From 2009.igem.org
(Difference between revisions)
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==To do== | ==To do== | ||
| - | ;- Find the exact genetic circuit for Trp repressor <t><b><font size=8><span style="color:dark red"> Nath </font size> | + | ;- Find the exact genetic circuit for Trp repressor <t><b><font size=8><span style="color:dark red"> Nath </span></font size></b> |
;- Write an e-mail to Strickland et al to ask | ;- Write an e-mail to Strickland et al to ask | ||
Revision as of 18:18, 26 May 2009
To find
- Sequence of the 12 mutant fusion proteins or LOV domain AND TrpR (separately) => PUBMED (http://www.ncbi.nlm.nih.gov/)
To do
- - Find the exact genetic circuit for Trp repressor <t> Nath
- - Write an e-mail to Strickland et al to ask
Sequence (of all 12 fusion proteins)
Purification protocol
Digest assay protocol
- - Biobricks
Look for a (or many) paper(s) that characterizes E.Coli Trp repressor, and find the Trp operon sequence
Design a biobrick that coexpresses LOVTAP and mCherry (after Trp operon) when LOVTAP bind the Trp operon. Design a switch on/off read out.
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