"
Virginia Commonwealth/4 August 2009
From 2009.igem.org
(Difference between revisions)
(→Tuesday 4 August 2009) |
(→Tuesday 4 August 2009) |
||
| Line 5: | Line 5: | ||
** Sample taken from plate after PSB4C5 ligation attempt successfully grew expressing RFP. The color of the sample was pink | ** Sample taken from plate after PSB4C5 ligation attempt successfully grew expressing RFP. The color of the sample was pink | ||
** Sample taken from plate after ligation with itself did not grow at all, and expressed no RFP | ** Sample taken from plate after ligation with itself did not grow at all, and expressed no RFP | ||
| - | [[User:Trentay|Trentay]] 22: | + | * There is a possibility the pSB4C5 backbone may be a bad part |
| + | ** previous years it has not functioned properly | ||
| + | ** Gel electrophoresis indicates DNA Gel Purification may be necessary | ||
| + | * Possible inefficiency of BioBrick enzymes may have come from repeated freezing and thawing | ||
| + | ** They will be aliquoted into smaller amounts | ||
| + | [[User:Trentay|Trentay]] 22:25, 4 August 2009 (UTC) | ||
---- | ---- | ||
===Tasks=== | ===Tasks=== | ||
| Line 24: | Line 29: | ||
''Maria and Afton'' | ''Maria and Afton'' | ||
| - | * | + | * Gel Electrophoresis was run |
| + | ** pSB4C5 and I1352 digested and undigested (right after Miniprep) were run on a gel and results showed excessive bands in the undigested DNA. DNA Gel Purification will be done on future Miniprepped DNA | ||
Revision as of 22:25, 4 August 2009
Contents |
Tuesday 4 August 2009
Results
Maria and Afton
- Overnight culture of I1352 plated on AMP:
- Sample taken from plate after PSB4C5 ligation attempt successfully grew expressing RFP. The color of the sample was pink
- Sample taken from plate after ligation with itself did not grow at all, and expressed no RFP
- There is a possibility the pSB4C5 backbone may be a bad part
- previous years it has not functioned properly
- Gel electrophoresis indicates DNA Gel Purification may be necessary
- Possible inefficiency of BioBrick enzymes may have come from repeated freezing and thawing
- They will be aliquoted into smaller amounts
Trentay 22:25, 4 August 2009 (UTC)
Tasks
Maria and Afton
- Run a Gel Electrophoresis
- Transform and grow up pSB1C3 with the death gene P1010
Trentay 22:11, 4 August 2009 (UTC)
Wetlab
- New BioBrick Assembly Kit came in and the following were aliquoted into their respective amounts
- XbaI (10 µL)
- SpeI (5 µL)
- PstI (10 µL)
- EcoRI-HF (10 µL)
- BSA (5 µL)
- T4 Ligase Buffer (10 µL)
- T4 Ligase (5 µL)
Maria and Afton
- Gel Electrophoresis was run
- pSB4C5 and I1352 digested and undigested (right after Miniprep) were run on a gel and results showed excessive bands in the undigested DNA. DNA Gel Purification will be done on future Miniprepped DNA
