Team:Paris/Freezer PCR products

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(Difference between revisions)
(→PCR products)
(→PCR products)
Line 55: Line 55:
|width=10%| [O19] g3p_F  
|width=10%| [O19] g3p_F  
|width=10%| [O20] g3p_R
|width=10%| [O20] g3p_R
-
|width=5%| Tm
+
|width=5%| 78°C
-
|width=10%| Matrix
+
|width=10%| P12
-
|width=10%| Length
+
|width=10%| 1287bp
|width=25%| For standardisation of g3p and adding SacI site
|width=25%| For standardisation of g3p and adding SacI site
|width=5%| ?
|width=5%| ?
Line 68: Line 68:
|width=10%| [O22] Ompa_linker_R
|width=10%| [O22] Ompa_linker_R
|width=5%| 80°C
|width=5%| 80°C
-
|width=10%| Matrix
+
|width=10%| P6
|width=10%| 525bp
|width=10%| 525bp
|width=25%| For standardisation of OmpA linker (BBaK103006)  
|width=25%| For standardisation of OmpA linker (BBaK103006)  

Revision as of 23:35, 4 August 2009

Freezer

Primers - PCR products - Plasmids - Digestion Products - Unexpected digestion results - Strains (Glycerol stock)


Color code for sticker
Vector Insert PCR product Plasmid Strains Kanamycin Ampicilin Chloranphenicol

PCR products

PCR products
Number Name Oligo F Oligo R Tm Matrix Length Comments Quantity []ng/µL Date
A1 g3p_std [O19] g3p_F [O20] g3p_R 78°C P12 1287bp For standardisation of g3p and adding SacI site  ?  ? ng/µL  ?
A2 OmpA_L_std [O21] Ompa_linker_F [O22] Ompa_linker_R 80°C P6 525bp For standardisation of OmpA linker (BBaK103006)  ?  ? ng/µL  ?



http://parts.mit.edu/r/parts/images/partbypart/icon_terminator.png http://parts.mit.edu/images/partbypart/icon_regulatory.png http://parts.mit.edu/r/parts/images/partbypart/icon_rbs.png http://parts.mit.edu/r/parts/images/partbypart/icon_reporter.png http://parts.mit.edu/registry/images/7/7b/Part_icon_cds.png http://parts.mit.edu/registry/images/9/9f/Part_icon_dna.png http://parts.mit.edu/registry/images/2/2e/Part_icon_generator.png

http://parts.mit.edu/registry/images/a/ab/Part_icon_plasmid.png

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