Revision as of 09:41, 7 August 2009

Used Parts
[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=KULeuven Submitted Parts]

Blue light receptor

part	plasmid	description	status	μl left	concentration	date
MC4100	/	strain of E.coli				july 24
2171	/	forward primer for		90µl	20μM	july 24
2172	/	reverse primer for		90µl	20μM	july 24
2171	/	forward primer for			100μM	july 24
2172	/	reverse primer for			100μM	july 24
	pSB1A2	GFP + rbs + terminator	purified			july 29
	pSB1A2	GFP + rbs + terminator	restricted with EcoRI and XbaI			july 29
	/	BLR promoter region	restricted with EcoRI			july 29
	/	BLR promoter region	4 microcentrifuge tubes			july 30

part	plasmid	description	status	μl left	concentration	date
	/	GFP with LVA tag	purified			july 28
	/	tetR promoter	purified			july 28

plasmid	description	status	concentration	date
pSB1A2	ech + rbs	purified plasmid	108,8 ng/µl	August 4
pSB1A2	fcs + rbs	purified plasmid	113,9 ng/µl	August 4
pSB1A2	Sam5 + rbs	received from Edinburgh		August 3
pSB1A2	Sam5 + rbs, PstI site removed	purified plasmid	101,3 ng/µl	August 5
pSB1A2	Sam8 + rbs	purified plasmid	95,7 ng/µl	August 5
pSB1A2	Sam8	purified plasmid	81,5 ng/µl	August 5
pSB1A2	COMT	purified plasmid	122,9 ng/µl	August 5
pSB1A2	COMT + rbs	plasmid from registry		August 6
pSB1A2	ech + rbs	purified digest	16,9 ng/µl	August 6
	fcs + rbs	purified digest	16,6 ng/µl	August 6
pSB1A2	sam5 + rbs	purified digest	3,1 ng/µl	August 7
	sam8 + rbs	purified digest	3,4 ng/µl	August 7

part	plasmid	description	status	μl left	concentration	date

part	plasmid	description	μl left	concentration	date
dNTP mix		ATP, GTP, TTP, CTP	94	10μM	July 24
	pSB1A2	medium constitutieve promoter			July 23
ampicilline

@@ Line 77: / Line 77: @@
 |-
 |||||fcs + rbs||purified digest||||16,6 ng/µl||August 6
+|-
+|||pSB1A2||sam5 + rbs||purified digest||||3,1 ng/µl||August 7
+|-
+|||||sam8 + rbs||purified digest||||3,4 ng/µl ||August 7
 |}