"
Ann's Notebook
From 2009.igem.org
(Difference between revisions)
| Line 48: | Line 48: | ||
[[media:8_5_09_Pu_promoter_colony_PCR_optimization,_Pu_promoter_purification_and_nanodrop.pdf| 8/5/09]] | [[media:8_5_09_Pu_promoter_colony_PCR_optimization,_Pu_promoter_purification_and_nanodrop.pdf| 8/5/09]] | ||
*Protocol for DNA purification | *Protocol for DNA purification | ||
| + | *DNA purification of Pu promoter colony PCR | ||
*Optimization of Pu promoter colony PCR | *Optimization of Pu promoter colony PCR | ||
*Nanodrop of Pu promoter | *Nanodrop of Pu promoter | ||
Revision as of 18:27, 6 August 2009
- Media preparation for transformation
- Comp. cell protocol
- Protocol for getting parts from the biobricks
- Transformation protocol
- Transformation of RBS for Pu promoter, Bacteriophage lysis cassette, lambda lysis device, T4 lysis device
- Protocol for miniprep
- Miniprep of RBS for Pu promoter, Bacteriophage lysis cassette, lambda lysis device, T4 lysis device
- Protocol for using the nanodrop
- Protocol for a digestion
- Nanodrop concentrations of the RBS for Pu promoter, Bacteriophage lysis cassette, lambda lysis device, T4 lysis device
- Digestion of the RBS for Pu promoter and the Bacteriophage lysis cassette
- Plated overnight cultures for Pu colony PCR
- Protocol for running a gel
- Ran gel of the RBS for Pu promoter and Bacteriophage lysis cassette
- Plated Pu-luciferase part sent from the registry
- Overnight culture for frozen stock of Pu-luciferase biobrick
- Digest of lambda and T4 killer genes to check part length with EcoRI, PstI, BglII and EcoRI/BglII
- Frozen stock of Pu-luciferase biobrick
- Gel of lambda and T4 killer genes digested with EcoRI, PstI, BglII and EcoRI/BglII
- Digest of lambda and T4 killer genes with EcoRI/PstI, EcoRI/XhoI, XhoI
- Gel of lambda and T4 killer genes digested with EcoRI/PstI, EcoRI/XhoI, XhoI
- Gel of Pu promoter colony PCR
- Analysis of Pu promoter colony PCR
- Primer sequence
- PCR conditions
- Protocol for colony PCR
- Colony PCR for Pu Promoter
- Gel of Colony PCR for Pu promoter with samples at different annealing temperatures
- Meeting with Pablo about HPLC
- Protocol for DNA purification
- DNA purification of Pu promoter colony PCR
- Optimization of Pu promoter colony PCR
- Nanodrop of Pu promoter
