August/6 August 2009

From 2009.igem.org

(Difference between revisions)
(Design genetic circuits)
(Design genetic circuits)
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We take notice of signal molecules. So I put them in order in the chart below.<br/>
We take notice of signal molecules. So I put them in order in the chart below.<br/>
                                                                                                                                 Indcer  signal moleculer  Receiver  
                                                                                                                                 Indcer  signal moleculer  Receiver  
-
  LuxI ⇒     3OC6HSL      ⇒ LuxR
+
* LuxI ⇒     3OC6HSL      ⇒ LuxR
-
  LasI ⇒  AI-1(3OC12HSL)  ⇒ LasR
+
LasI ⇒  AI-1(3OC12HSL)  ⇒ LasR
-
  CinI  ⇒   3OH,C14:1-HSl   ⇒ CinR
+
* CinI  ⇒   3OH,C14:1-HSl   ⇒ CinR
-
  RhlI ⇒ 
+
* RhlI ⇒ AI-1(C4HSL)  ⇒ RhlR

Revision as of 22:08, 6 August 2009

Contents

Morning Meeting

To do in lab

1. Transform & Selection

  • 培地作り
    • LB
    • LB Amp+
    • LB Kan+
  • Transformation
    • About 16 kinds of parts

2. Breeding

3. Refinement

To bring

  • Timer
  • Pen
  • Slipper

Design genetic circuits

We take notice of signal molecules. So I put them in order in the chart below.
  Indcer signal moleculer Receiver

* LuxI ⇒     3OC6HSL       ⇒ LuxR
*  LasI ⇒  AI-1(3OC12HSL)  ⇒ LasR
* CinI  ⇒   3OH,C14:1-HSl   ⇒ CinR
* RhlI ⇒ AI-1(C4HSL)   ⇒ RhlR