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August/6 August 2009
From 2009.igem.org
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(→Design genetic circuits) |
(→Design genetic circuits) |
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From now on,We have to do what I mention below | From now on,We have to do what I mention below | ||
| - | + | 1.Search other cell-cell comunication system | |
| - | + | 2.Check systems component completely | |
| - | + | 3.Create new systems or reinforce cell funcution with additional ideas | |
| - | + | 4.Search the effect of cross talk | |
written by Tadasi Nakamura | written by Tadasi Nakamura | ||
Revision as of 22:54, 6 August 2009
Contents |
Morning Meeting
To do in lab
1. Transform & Selection
- 培地作り
- LB
- LB Amp+
- LB Kan+
- Transformation
- About 16 kinds of parts
2. Breeding
3. Refinement
To bring
- Timer
- Pen
- Slipper
Design genetic circuits
We take notice of signal molecules. So I put them in order in the chart below.
*Inducer ⇒（synthesize) signal moleculer ⇒（synthesize) Receiver
* LuxI ⇒ 3OC6HSL ⇒ LuxR * LasI ⇒ AI-1(3OC12HSL) ⇒ LasR * CinI ⇒ 3OH,C14:1-HSl ⇒ CinR * RhlI ⇒ AI-1(C4HSL) ⇒ RhlR * （exception）agrD synthesize AIP with agrB.AIp synthesize agrC . AgrA which is synthesized by agrC activate promoter.
From now on,We have to do what I mention below
1.Search other cell-cell comunication system
2.Check systems component completely
3.Create new systems or reinforce cell funcution with additional ideas
4.Search the effect of cross talk
written by Tadasi Nakamura
