Revision as of 07:32, 21 August 2009

Morning Meeting

To do in lab

1. Transform & Selection

Medium buildng
- LB
- LB Amp+
- LB Kan+
Transformation
- About 16 kinds of parts

   We transform these following 14 parts today.
      B0015 Plate 1 23L    S03878 Plate2 16M
      C0179 Pate 2 8M     C0070 Plate2  12H
      F1610 Plate2 24G     B0034 Plate1 2M
      I0462 Plate1 8O      C0078 Plate1 14D
      C0077 Plate1 14A    I1466 Plate1 23J

2. Breeding

3. Refinement

To bring

Timer
Pen
Slipper

Design genetic circuits

We have thus far focused our attention on signaling molecules used in quorum sensing systems of bacteria. The ones we will be using are as shown below:
　 *Inducer ⇒（synthesize) Signal molecule ⇒ Receptor

* LuxI ⇒　    3OC6HSL　     　⇒ LuxR → positive regulation
* LasI ⇒　　AI-1(3OC12HSL) 　⇒　LasR → positive regulation
* CinI  ⇒  　3OH,C14:1-HSl  　⇒　CinR → positive regulation
* RhlI ⇒　　AI-1(C4HSL)  　　 ⇒　RhlR → positive regulation
* AgrB synthesizes AIP from AgrD ⇒ AIP diffuses and attaches to AgrC ⇒ AgrC phosphorylates 
and activates AgrA which then promotes transcription at P2 and P3 promoters.

 From now on,We have to do what I mention below
 1.Investigate other cell-cell comunication systems (besides those used in quorum-sensing).
 2.Check to ensure that system components are working properly.
 3.Create new systems or implement additional cell functions.
 4.Investigate the possibility and extent of cross talk between various signaling molecules.

          reported by Tadasi Nakamura

August/6 August 2009

From 2009.igem.org

Revision as of 07:32, 21 August 2009

Contents

Morning Meeting

To do in lab

To bring

Design genetic circuits